篩檢血液中循環腫瘤細胞之方法

The present invention relates to a method which uses a blood cell removal technique together with a multi-parameter flow cytometry to detect and quantify circulating tumor cells in blood. The method includes: obtaining blood which contains suspicious rare cell mixes from a testing object; removing red blood cells and white blood cells which may interfere the analysis, and adding at least one fluorescent antibody for identifying rare cells into the blood; using the multi-parameter flow cytometry to detect, qualify, purify, and separate the circulating tumor cells that show fluorescent conjugated specific antibodies; and finally, collecting the circulating tumor cells that include the fluorescent conjugated specific antibodies. The method improves a detection rate of circulating tumor cells in blood, shortens analysis time, and accelerates cancer screening and recovery assessment.