表面電漿波相位生物感知器對中藥抑制貝它澱粉樣蛋白(Aβ)聚合之評估

阿滋海默氏症患者腦部散佈著類澱粉沈積（amyloid deposits）的病理特徵。類澱粉沈積的主要成分為A?（?-amyloid protein）。 透過表面電漿生物感知器快速、即時觀察、不需標示（labeling）的優點，我們可以即時觀察並記錄生物分子間的相互作用情形而進行動力學之分析，提供對早期A?纖維束形成動力學的研究。 以抑制劑抑制A?1-42凝集反應中，發現nicotine及銀杏粗萃物EGb761，可分別在凝集反應及解離反應中，抑制A?1-42的聚合並促進A?1-42凝集物解離。
Substantial evidence indicates that aggregation of ?-amyloid (A?) peptides resulting neuronal toxicity probably play a causative role in the etiology of AD (Alzheimer's disease). Past studies of the kinetics of A? polymerization, could only provide information on the appearance of high molecular weight aggregates. Rate constants could not be detemined by these approaches. In contrast, surface plasmon resonance (SPR) biosensor can be used to monitor binding events in real time without labeling, making them convenient for studying early-stage A? polymerization. Using nicotine and extract of Gingko biloba, EGb761, as inhibitors, it has been shown that both of them can retard A?1-42 polymerization and promote dissociation in the association and dissociation phase.