過敏性鼻炎針刺治療前後之微陣列分析

基因微陣列（Microarray）為近年來新發展的分類與鑑定的工具，本計畫微陣列實驗由長庚醫院及長庚大學的「基因體醫學研究核心實驗室」（Genomic Medicine Research Core Laboratory-GMRCL），進行「過敏性鼻炎針刺治療前後之微陣列分析」，利用血液檢體進行cDNA microarray，以測量針刺治療前後之免疫反應基因與過敏性鼻炎臨床資料的相關性。本計畫將探討針刺對過敏性鼻炎的分子生物學與免疫學上的機轉，患者進行針刺治療，在治療過程中，抽取病人血液檢體為材料，進行microarray以測量免疫反應基因的變化。 臨床上許多過敏性鼻炎患者，因為畏懼西醫藥物治療的副作用，而求助於傳統中醫藥以及針刺的幫助，針刺具有良好的治療與保健作用，從免疫的角度對針刺作用探討研究顯示，針刺可以調整體內各種不同的免疫物質趨于穩定和平衡，使之有利于人體生理機能，近年已有研究指出，以針刺治療氣喘或過敏性鼻炎，可以有效改善免疫反應。本計畫希望藉由結合中醫部門臨床醫師與GMRCL實驗室的豐富Microarray經驗，更深入探討針刺對過敏性鼻炎的作用機轉。以基因導向醫學觀點檢驗傳統中醫學常見之「同病異治」與「異病同治」。 預計針對18-45歲出現打噴嚏、流鼻水及鼻塞等三大症狀的患者，且治療前一個月未曾服用中藥或西藥者，給予針刺治療。病患將接受為期4週、每週2次，總計共8次的針刺治療。並在針刺前、針刺後2小時、針刺後24小時與接受第八次針刺療程後，對病患抽血（總計進行4次，每次抽血5mL），進行周邊血液分析以及Microarray分析。Microarray結果分析將研究：（I）過敏性鼻炎患者針刺治療前後，血液中基因表現的改變。（II）血液中基因表現的改變與特定血球數量的關連性。最後將會針對病患在治療前、中、後之臨床症狀做統計分析，並與Microarray結果分析做關聯對照，以獲得最後之結論。
This project “The cDNA Microarray analysis for allergic rhinitis treatment in Traditional Chinese Medicine” will be performed at Genomic Medicine Research Core Laboratory-GMRCL in Chung Gang University and Chung Gang memoral hospital. Allergic rhinitis patient will receive acupuncture treatment, blood sample was attained before and after the acupuncture therapy. Then we use cDNA microarray to analysis the immunomodulatory effects of acupuncture in the treatment of allergic rhinitie. Many allergic rhinitis patients receive tradition Chinese medicine treatment, to avoid the side effect of anti-histamine. Acupuncture treatment is a suitable treatment for complex chronic disease such an allergic rhinitis. According to recent journal reports, acupuncture performed in accordance with the principles of traditional Chinese medicine would show significant immune-modulating effects. This project plan to combine the experience of clinical doctor and GMRCL cDNA Microarray for the mechanism of acupuncture on allergic rhinitis. The principles of traditional Chinese medicine such as “treating the same disease with different therapies”, “treating different diseases with the same therapy” will be studied. We plan to study the effects of acupuncture treatment for allergic rhinitis. All patient during 18-45 years old were treated 8 times for 20 minutes over a time period of 4 weeks. Patients' general well-being and several peripheral blood parameters were determined before and after the acupuncture treatment. The cDNA microarray analysis would find the genomic change before and after the acupuncture treatment.