酸擴增方法及其裝置暨核酸檢測方法與其裝置

The invention relates to a method and device for nucleic acid amplification and amplicon detection thereof. The nucleic acid amplification device comprises a reaction unit, an energy excitation unit, and an operation unit, and an auxiliary cooling unit. The reaction unit includes test analytes, a solution for nucleic acid amplification, and one or more solid phase carriers, each of which is functionalized with specific surface ligands for capturing target analytes and further nucleic acid amplification. By controlling the excitation energy outputs and the opening and closing sequence of the energy excitation unit, whereby an isolated environment is formed and an ultrafast thermal cycling cycle is generated around solid phase carriers. The present invention uses this device to perform a nucleic acid amplification method, and also uses a nucleic acid detection method and its device to detect amplicons on one or more solid phase carriers.