2-O-methyl PAF as a Ca2+ mobilizer in Madin Darby canine kidney cells

Jeng Hsien Yeh, Chun Jen Huang, Jang Hwa Lee, Shu Shong Hsu, Jin Shyr Chen, He Hsiung Cheng, Hong Tai Chang, Jong Khing Huang, Hsiao Min Chung, Yeh Mei-Yin, Chung Ren Jan*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

2 Scopus citations

Abstract

In Madin-Darby canine kidney (MDCK) cells, the effect of 2-O-methyl PAF, an inactive analogue of platelet activating factor (PAF), on intracellular Ca 2+ concentration ([Ca2+]i) was measured by using the Ca2+-sensitive fluorescent dye fura-2. 2-O-methyl PAF (≥15 μM) caused a rapid rise of [Ca2+]i in a concentration-dependent manner. 2-O-methyl PAF-induced [Ca2+] i rise was partly reduced by removal of extracellular Ca 2+. 2-O-methyl PAF-induced extracellular Ca2+ influx was also suggested by Mn2+ influx-induced fura-2 fluorescence quench. The 2-O-methyl PAF-induced Ca2+ influx was blocked by nifedipine, verapamil and diltiazem. In Ca2+-free medium, thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+-ATPase, caused a monophasic [Ca2+]i rise, after which 2-O-methyl PAF failed to increase [Ca2+]i; also, pretreatment with 2-O-methyl PAF depleted thapsigargin-sensitive Ca2+ stores. U73122, an inhibitor of phospholipase C, abolished ATP (but not 2-O-methyl PAF)-induced [Ca 2+]i rise. These findings suggest that 2-O-methyl PAF evokes a rapid increase in [Ca2+]i in renal tubular cells by stimulating both extracellular Ca2+ influx and intracellular Ca2+ release.

Original languageEnglish
Pages (from-to)336-344
Number of pages9
JournalLife Sciences
Volume77
Issue number3
DOIs
StatePublished - 03 06 2005
Externally publishedYes

Keywords

  • 2-O-methyl PAF
  • Ca
  • Ca stores
  • Fura-2
  • Madin Darby canine kidney cells

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