A bioluminescent method for measuring thymidylate kinase activity suitable for high-throughput screening of inhibitor

Chun Mei Hu, Zee Fen Chang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

7 Scopus citations

Abstract

Blocking human thymidylate kinase (TMPK) function has a chemosensitization effect in anticancer treatment. However, a rapid and sensitive TMPK activity assay method suitable for inhibitor screening has been lacking. We have designed a luciferase-coupled TMPK assay in which luminescence emission is proportional to the magnitude of TMPK inhibition. The advantages of using this new method over the conventional nicotinamide adenine dinucleotide (reduced form, NADH)-coupling method in screening inhibitor include low cost, low limit in detecting inhibitory signal, more accurate, and devoid of interference due to compound absorbance at 340 nm.

Original languageEnglish
Pages (from-to)269-271
Number of pages3
JournalAnalytical Biochemistry
Volume398
Issue number2
DOIs
StatePublished - 03 2010
Externally publishedYes

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