TY - JOUR
T1 - A bioluminescent method for measuring thymidylate kinase activity suitable for high-throughput screening of inhibitor
AU - Hu, Chun Mei
AU - Chang, Zee Fen
PY - 2010/3
Y1 - 2010/3
N2 - Blocking human thymidylate kinase (TMPK) function has a chemosensitization effect in anticancer treatment. However, a rapid and sensitive TMPK activity assay method suitable for inhibitor screening has been lacking. We have designed a luciferase-coupled TMPK assay in which luminescence emission is proportional to the magnitude of TMPK inhibition. The advantages of using this new method over the conventional nicotinamide adenine dinucleotide (reduced form, NADH)-coupling method in screening inhibitor include low cost, low limit in detecting inhibitory signal, more accurate, and devoid of interference due to compound absorbance at 340 nm.
AB - Blocking human thymidylate kinase (TMPK) function has a chemosensitization effect in anticancer treatment. However, a rapid and sensitive TMPK activity assay method suitable for inhibitor screening has been lacking. We have designed a luciferase-coupled TMPK assay in which luminescence emission is proportional to the magnitude of TMPK inhibition. The advantages of using this new method over the conventional nicotinamide adenine dinucleotide (reduced form, NADH)-coupling method in screening inhibitor include low cost, low limit in detecting inhibitory signal, more accurate, and devoid of interference due to compound absorbance at 340 nm.
UR - http://www.scopus.com/inward/record.url?scp=77649182324&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2009.12.001
DO - 10.1016/j.ab.2009.12.001
M3 - 文章
C2 - 19995545
AN - SCOPUS:77649182324
SN - 0003-2697
VL - 398
SP - 269
EP - 271
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -