A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization

Song Bin Huang; Yang Zhao; Deyong Chen; Yana Luo; Hsin Chieh Lee; Tzu Keng Chiu; Junbo Wang; Jian Chen; Min Hsien Wu

doi:10.1109/NEMS.2014.6908836

A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization

Song Bin Huang
, Yang Zhao
, Deyong Chen
, Yana Luo
, Hsin Chieh Lee
, Tzu Keng Chiu
, Junbo Wang^*
, Jian Chen
, Min Hsien Wu

^*Corresponding author for this work

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This study reports a microfluidic platform for clogging-free electrical property analysis of cancer cells. A pneumatically-driven membrane-based active valve was integrated in this platform to unblock clogging events of constriction microchannels where pneumatic pressures were used to tune the deformation of a polydimethylsiloxan (PDMS) membrane serving as one wall of the constriction microchannel. The proposed platform was first used to unblock trapped polystyrene beads (30 μm in diameter) at the entrance of constriction microchannels and then the characterization of the cellular electrical properties of lung cancer cells was successfully demonstrated. Results showed that the measured cytoplasm conductivity (0.74±0.20 S/m) and specific membrane capacitance (2.17±0.58 μF/cm²) of cells were consistent with the results from the previous publications (0.73±0.17 S/m, and 2.00±0.60 μF/cm², respectively). Overall, this study has presented a microfluidic platform for single cell analysis with an enhanced function for unblocking cell aggregates at the entrance of microchannels, which may function as a platform technology enabling cancer-cell electrical property characterization in the near future.

Original language	English
Title of host publication	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	398-402
Number of pages	5
ISBN (Electronic)	9781479947270
DOIs	https://doi.org/10.1109/NEMS.2014.6908836
State	Published - 23 09 2014
Event	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 - Waikiki Beach, United States Duration: 13 04 2014 → 16 04 2014

Publication series

Name	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

Conference

Conference	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
Country/Territory	United States
City	Waikiki Beach
Period	13/04/14 → 16/04/14

Bibliographical note

Publisher Copyright:
© 2014 IEEE.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1109/NEMS.2014.6908836

Cite this

Huang, S. B., Zhao, Y., Chen, D., Luo, Y., Lee, H. C., Chiu, T. K., Wang, J., Chen, J., & Wu, M. H. (2014). A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization. In 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 (pp. 398-402). Article 6908836 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2014.6908836

Huang, Song Bin ; Zhao, Yang ; Chen, Deyong et al. / A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization. 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 398-402 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014).

@inproceedings{fdfa5329408c4415acab1fbc1a2e7d0c,

title = "A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization",

abstract = "This study reports a microfluidic platform for clogging-free electrical property analysis of cancer cells. A pneumatically-driven membrane-based active valve was integrated in this platform to unblock clogging events of constriction microchannels where pneumatic pressures were used to tune the deformation of a polydimethylsiloxan (PDMS) membrane serving as one wall of the constriction microchannel. The proposed platform was first used to unblock trapped polystyrene beads (30 μm in diameter) at the entrance of constriction microchannels and then the characterization of the cellular electrical properties of lung cancer cells was successfully demonstrated. Results showed that the measured cytoplasm conductivity (0.74±0.20 S/m) and specific membrane capacitance (2.17±0.58 μF/cm2) of cells were consistent with the results from the previous publications (0.73±0.17 S/m, and 2.00±0.60 μF/cm2, respectively). Overall, this study has presented a microfluidic platform for single cell analysis with an enhanced function for unblocking cell aggregates at the entrance of microchannels, which may function as a platform technology enabling cancer-cell electrical property characterization in the near future.",

author = "Huang, \{Song Bin\} and Yang Zhao and Deyong Chen and Yana Luo and Lee, \{Hsin Chieh\} and Chiu, \{Tzu Keng\} and Junbo Wang and Jian Chen and Wu, \{Min Hsien\}",

note = "Publisher Copyright: {\textcopyright} 2014 IEEE.; 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 ; Conference date: 13-04-2014 Through 16-04-2014",

year = "2014",

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day = "23",

doi = "10.1109/NEMS.2014.6908836",

language = "英语",

series = "9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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}

Huang, SB, Zhao, Y, Chen, D, Luo, Y, Lee, HC, Chiu, TK, Wang, J, Chen, J & Wu, MH 2014, A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization. in 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014., 6908836, 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014, Institute of Electrical and Electronics Engineers Inc., pp. 398-402, 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014, Waikiki Beach, United States, 13/04/14. https://doi.org/10.1109/NEMS.2014.6908836

A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization. / Huang, Song Bin; Zhao, Yang; Chen, Deyong et al.
9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. p. 398-402 6908836 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization

AU - Huang, Song Bin

AU - Zhao, Yang

AU - Chen, Deyong

AU - Luo, Yana

AU - Lee, Hsin Chieh

AU - Chiu, Tzu Keng

AU - Wang, Junbo

AU - Chen, Jian

AU - Wu, Min Hsien

PY - 2014/9/23

Y1 - 2014/9/23

N2 - This study reports a microfluidic platform for clogging-free electrical property analysis of cancer cells. A pneumatically-driven membrane-based active valve was integrated in this platform to unblock clogging events of constriction microchannels where pneumatic pressures were used to tune the deformation of a polydimethylsiloxan (PDMS) membrane serving as one wall of the constriction microchannel. The proposed platform was first used to unblock trapped polystyrene beads (30 μm in diameter) at the entrance of constriction microchannels and then the characterization of the cellular electrical properties of lung cancer cells was successfully demonstrated. Results showed that the measured cytoplasm conductivity (0.74±0.20 S/m) and specific membrane capacitance (2.17±0.58 μF/cm2) of cells were consistent with the results from the previous publications (0.73±0.17 S/m, and 2.00±0.60 μF/cm2, respectively). Overall, this study has presented a microfluidic platform for single cell analysis with an enhanced function for unblocking cell aggregates at the entrance of microchannels, which may function as a platform technology enabling cancer-cell electrical property characterization in the near future.

AB - This study reports a microfluidic platform for clogging-free electrical property analysis of cancer cells. A pneumatically-driven membrane-based active valve was integrated in this platform to unblock clogging events of constriction microchannels where pneumatic pressures were used to tune the deformation of a polydimethylsiloxan (PDMS) membrane serving as one wall of the constriction microchannel. The proposed platform was first used to unblock trapped polystyrene beads (30 μm in diameter) at the entrance of constriction microchannels and then the characterization of the cellular electrical properties of lung cancer cells was successfully demonstrated. Results showed that the measured cytoplasm conductivity (0.74±0.20 S/m) and specific membrane capacitance (2.17±0.58 μF/cm2) of cells were consistent with the results from the previous publications (0.73±0.17 S/m, and 2.00±0.60 μF/cm2, respectively). Overall, this study has presented a microfluidic platform for single cell analysis with an enhanced function for unblocking cell aggregates at the entrance of microchannels, which may function as a platform technology enabling cancer-cell electrical property characterization in the near future.

UR - https://www.scopus.com/pages/publications/84908658707

U2 - 10.1109/NEMS.2014.6908836

DO - 10.1109/NEMS.2014.6908836

M3 - 会议稿件

AN - SCOPUS:84908658707

T3 - 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

SP - 398

EP - 402

BT - 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

Y2 - 13 April 2014 through 16 April 2014

ER -

Huang SB, Zhao Y, Chen D, Luo Y, Lee HC, Chiu TK et al. A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization. In 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc. 2014. p. 398-402. 6908836. (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014). doi: 10.1109/NEMS.2014.6908836

A clogging-free microfluidic platform for size independent single cancer cellular electrical property characterization

Abstract

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Bibliographical note

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