A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS

Tsung Hsien Wu; Yi Sin Chen; Huey Ling You; Mel S. Lee; Tien Tsai Cheng; Gwo Bin Lee

A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS

Tsung Hsien Wu
, Yi Sin Chen
, Huey Ling You
, Mel S. Lee
, Tien Tsai Cheng
, Gwo Bin Lee^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Anti-cyclic citrullinated peptide antibody (anti-CCP) and rheumatoid factors (RF) have been proven to be reliable biomarkers for rheumatoid arthritis (RA) diagnosis due to their high sensitivity and specificity. However, current approaches are relatively time-consuming and labor-intensive. Hence, this study demonstrated a new pneumatically-driven microfluidic platform to carry out on-chip enzyme-linked immunosorbent assays by using antigen-coated magnetic beads, horseradish peroxidase conjugated antibodies and tetramethylbenzidine for quantification of two target biomarkers on one chip from serum simultaneously. This new platform could automatically perform dual-target diagnosis within 55 min while consuming only 50 μL of samples, showing a great potential to advance RA diagnosis for clinical use.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1161-1162
Number of pages	2
ISBN (Electronic)	9781733419031
State	Published - 2021
Externally published	Yes
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10 10 2021 → 14 10 2021

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21

Bibliographical note

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

Anti-cyclic citrullinated peptide
Enzyme-linked immunosorbent assay
Microfluidics
Rheumatoid arthritis
Rheumatoid factors

Cite this

Wu, T. H., Chen, Y. S., You, H. L., Lee, M. S., Cheng, T. T., & Lee, G. B. (2021). A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1161-1162). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Wu, Tsung Hsien ; Chen, Yi Sin ; You, Huey Ling et al. / A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 1161-1162 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{f09ecb795db1447d944a10cd3bbe590d,

title = "A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS",

abstract = "Anti-cyclic citrullinated peptide antibody (anti-CCP) and rheumatoid factors (RF) have been proven to be reliable biomarkers for rheumatoid arthritis (RA) diagnosis due to their high sensitivity and specificity. However, current approaches are relatively time-consuming and labor-intensive. Hence, this study demonstrated a new pneumatically-driven microfluidic platform to carry out on-chip enzyme-linked immunosorbent assays by using antigen-coated magnetic beads, horseradish peroxidase conjugated antibodies and tetramethylbenzidine for quantification of two target biomarkers on one chip from serum simultaneously. This new platform could automatically perform dual-target diagnosis within 55 min while consuming only 50 μL of samples, showing a great potential to advance RA diagnosis for clinical use.",

keywords = "Anti-cyclic citrullinated peptide, Enzyme-linked immunosorbent assay, Microfluidics, Rheumatoid arthritis, Rheumatoid factors",

author = "Wu, \{Tsung Hsien\} and Chen, \{Yi Sin\} and You, \{Huey Ling\} and Lee, \{Mel S.\} and Cheng, \{Tien Tsai\} and Lee, \{Gwo Bin\}",

note = "Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

language = "英语",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "1161--1162",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

Wu, TH, Chen, YS, You, HL, Lee, MS, Cheng, TT & Lee, GB 2021, A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 1161-1162, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.

A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS. / Wu, Tsung Hsien; Chen, Yi Sin; You, Huey Ling et al.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 1161-1162 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS

AU - Wu, Tsung Hsien

AU - Chen, Yi Sin

AU - You, Huey Ling

AU - Lee, Mel S.

AU - Cheng, Tien Tsai

AU - Lee, Gwo Bin

PY - 2021

Y1 - 2021

N2 - Anti-cyclic citrullinated peptide antibody (anti-CCP) and rheumatoid factors (RF) have been proven to be reliable biomarkers for rheumatoid arthritis (RA) diagnosis due to their high sensitivity and specificity. However, current approaches are relatively time-consuming and labor-intensive. Hence, this study demonstrated a new pneumatically-driven microfluidic platform to carry out on-chip enzyme-linked immunosorbent assays by using antigen-coated magnetic beads, horseradish peroxidase conjugated antibodies and tetramethylbenzidine for quantification of two target biomarkers on one chip from serum simultaneously. This new platform could automatically perform dual-target diagnosis within 55 min while consuming only 50 μL of samples, showing a great potential to advance RA diagnosis for clinical use.

AB - Anti-cyclic citrullinated peptide antibody (anti-CCP) and rheumatoid factors (RF) have been proven to be reliable biomarkers for rheumatoid arthritis (RA) diagnosis due to their high sensitivity and specificity. However, current approaches are relatively time-consuming and labor-intensive. Hence, this study demonstrated a new pneumatically-driven microfluidic platform to carry out on-chip enzyme-linked immunosorbent assays by using antigen-coated magnetic beads, horseradish peroxidase conjugated antibodies and tetramethylbenzidine for quantification of two target biomarkers on one chip from serum simultaneously. This new platform could automatically perform dual-target diagnosis within 55 min while consuming only 50 μL of samples, showing a great potential to advance RA diagnosis for clinical use.

KW - Anti-cyclic citrullinated peptide

KW - Enzyme-linked immunosorbent assay

KW - Microfluidics

KW - Rheumatoid arthritis

KW - Rheumatoid factors

UR - https://www.scopus.com/pages/publications/85136934407

M3 - 会议稿件

AN - SCOPUS:85136934407

T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1161

EP - 1162

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Wu TH, Chen YS, You HL, Lee MS, Cheng TT, Lee GB. A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 1161-1162. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

A DUAL-TARGET MICROFLUIDIC PLATFORM FOR DIAGNOSIS OF RHEUMATOID ARTHRITIS

Abstract

Publication series

Conference

Bibliographical note

Keywords

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Cite this