A function for the AP3 coat complex in synaptic vesicle formation from endosomes

Victor Faúndez; Jim Tong Horng; Regis B. Kelly

doi:10.1016/S0092-8674(00)81170-8

A function for the AP3 coat complex in synaptic vesicle formation from endosomes

Victor Faúndez, Jim Tong Horng, Regis B. Kelly^*

^*Corresponding author for this work

University of California at San Francisco

Research output: Contribution to journal › Journal Article › peer-review

252 Scopus citations

Abstract

Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgito-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.

Original language	English
Pages (from-to)	423-432
Number of pages	10
Journal	Cell
Volume	93
Issue number	3
DOIs	https://doi.org/10.1016/S0092-8674(00)81170-8
State	Published - 01 05 1998
Externally published	Yes

Access to Document

10.1016/S0092-8674(00)81170-8

Cite this

@article{3137497423914e459a3b96620087bf1f,

title = "A function for the AP3 coat complex in synaptic vesicle formation from endosomes",

abstract = "Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgito-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.",

author = "Victor Fa{\'u}ndez and Horng, {Jim Tong} and Kelly, {Regis B.}",

year = "1998",

month = may,

day = "1",

doi = "10.1016/S0092-8674(00)81170-8",

language = "英语",

volume = "93",

pages = "423--432",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - A function for the AP3 coat complex in synaptic vesicle formation from endosomes

AU - Faúndez, Victor

AU - Horng, Jim Tong

AU - Kelly, Regis B.

PY - 1998/5/1

Y1 - 1998/5/1

N2 - Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgito-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.

AB - Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgito-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.

UR - http://www.scopus.com/inward/record.url?scp=0032076102&partnerID=8YFLogxK

U2 - 10.1016/S0092-8674(00)81170-8

DO - 10.1016/S0092-8674(00)81170-8

M3 - 文章

C2 - 9590176

AN - SCOPUS:0032076102

SN - 0092-8674

VL - 93

SP - 423

EP - 432

JO - Cell

JF - Cell

IS - 3

ER -

A function for the AP3 coat complex in synaptic vesicle formation from endosomes

Abstract

Access to Document

Other files and links

Fingerprint

Cite this