A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

Alain Townsend; Pramila Rijal; Julie Xiao; Tiong Kit Tan; Kuan Ying A. Huang; Lisa Schimanski; Jiandong Huo; Nimesh Gupta; Rolle Rahikainen; Philippa C. Matthews; Derrick Crook; Sarah Hoosdally; Susanna Dunachie; Eleanor Barnes; Teresa Street; Christopher P. Conlon; John Frater; Carolina V. Arancibia-Cárcamo; Justine Rudkin; Nicole Stoesser; Fredrik Karpe; Matthew Neville; Rutger Ploeg; Marta Oliveira; David J. Roberts; Abigail A. Lamikanra; Hoi Pat Tsang; Abbie Bown; Richard Vipond; Alexander J. Mentzer; Julian C. Knight; Andrew J. Kwok; Gavin R. Screaton; Juthathip Mongkolsapaya; Wanwisa Dejnirattisai; Piyada Supasa; Paul Klenerman; Christina Dold; J. Kenneth Baillie; Shona C. Moore; Peter J.M. Openshaw; Malcolm G. Semple; Lance C.W. Turtle; Mark Ainsworth; Alice Allcock; Sally Beer; Sagida Bibi; Donal Skelly; Lizzy Stafford; Katie Jeffrey; Denise O’Donnell; Elizabeth Clutterbuck; Alexis Espinosa; Maria Mendoza; Dominique Georgiou; Teresa Lockett; Jose Martinez; Elena Perez; Veronica Gallardo Sanchez; Giuseppe Scozzafava; Alberto Sobrinodiaz; Hannah Thraves; Etienne Joly

doi:10.1038/s41467-021-22045-y

A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

Alain Townsend^*, Pramila Rijal, Julie Xiao, Tiong Kit Tan, Kuan Ying A. Huang, Lisa Schimanski, Jiandong Huo, Nimesh Gupta, Rolle Rahikainen, Philippa C. Matthews, Derrick Crook, Sarah Hoosdally, Susanna Dunachie, Eleanor Barnes, Teresa Street, Christopher P. Conlon, John Frater, Carolina V. Arancibia-Cárcamo, Justine Rudkin, Nicole StoesserFredrik Karpe, Matthew Neville, Rutger Ploeg, Marta Oliveira, David J. Roberts, Abigail A. Lamikanra, Hoi Pat Tsang, Abbie Bown, Richard Vipond, Alexander J. Mentzer, Julian C. Knight, Andrew J. Kwok, Gavin R. Screaton, Juthathip Mongkolsapaya, Wanwisa Dejnirattisai, Piyada Supasa, Paul Klenerman, Christina Dold, J. Kenneth Baillie, Shona C. Moore, Peter J.M. Openshaw, Malcolm G. Semple, Lance C.W. Turtle, Mark Ainsworth, Alice Allcock, Sally Beer, Sagida Bibi, Donal Skelly, Lizzy Stafford, Katie Jeffrey, Denise O’Donnell, Elizabeth Clutterbuck, Alexis Espinosa, Maria Mendoza, Dominique Georgiou, Teresa Lockett, Jose Martinez, Elena Perez, Veronica Gallardo Sanchez, Giuseppe Scozzafava, Alberto Sobrinodiaz, Hannah Thraves, Etienne Joly^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

47 Scopus citations

Abstract

Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.

Original language	English
Article number	1951
Journal	Nature Communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-22045-y
State	Published - 01 12 2021

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Publisher Copyright:
© 2021, The Author(s).

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Townsend, A., Rijal, P., Xiao, J., Tan, T. K., Huang, K. Y. A., Schimanski, L., Huo, J., Gupta, N., Rahikainen, R., Matthews, P. C., Crook, D., Hoosdally, S., Dunachie, S., Barnes, E., Street, T., Conlon, C. P., Frater, J., Arancibia-Cárcamo, C. V., Rudkin, J., ... Joly, E. (2021). A haemagglutination test for rapid detection of antibodies to SARS-CoV-2. Nature Communications, 12(1), Article 1951. https://doi.org/10.1038/s41467-021-22045-y

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abstract = "Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.",

author = "Alain Townsend and Pramila Rijal and Julie Xiao and Tan, {Tiong Kit} and Huang, {Kuan Ying A.} and Lisa Schimanski and Jiandong Huo and Nimesh Gupta and Rolle Rahikainen and Matthews, {Philippa C.} and Derrick Crook and Sarah Hoosdally and Susanna Dunachie and Eleanor Barnes and Teresa Street and Conlon, {Christopher P.} and John Frater and Arancibia-C{\'a}rcamo, {Carolina V.} and Justine Rudkin and Nicole Stoesser and Fredrik Karpe and Matthew Neville and Rutger Ploeg and Marta Oliveira and Roberts, {David J.} and Lamikanra, {Abigail A.} and Tsang, {Hoi Pat} and Abbie Bown and Richard Vipond and Mentzer, {Alexander J.} and Knight, {Julian C.} and Kwok, {Andrew J.} and Screaton, {Gavin R.} and Juthathip Mongkolsapaya and Wanwisa Dejnirattisai and Piyada Supasa and Paul Klenerman and Christina Dold and Baillie, {J. Kenneth} and Moore, {Shona C.} and Openshaw, {Peter J.M.} and Semple, {Malcolm G.} and Turtle, {Lance C.W.} and Mark Ainsworth and Alice Allcock and Sally Beer and Sagida Bibi and Donal Skelly and Lizzy Stafford and Katie Jeffrey and Denise O{\textquoteright}Donnell and Elizabeth Clutterbuck and Alexis Espinosa and Maria Mendoza and Dominique Georgiou and Teresa Lockett and Jose Martinez and Elena Perez and {Gallardo Sanchez}, Veronica and Giuseppe Scozzafava and Alberto Sobrinodiaz and Hannah Thraves and Etienne Joly",

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doi = "10.1038/s41467-021-22045-y",

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Townsend, A, Rijal, P, Xiao, J, Tan, TK, Huang, KYA, Schimanski, L, Huo, J, Gupta, N, Rahikainen, R, Matthews, PC, Crook, D, Hoosdally, S, Dunachie, S, Barnes, E, Street, T, Conlon, CP, Frater, J, Arancibia-Cárcamo, CV, Rudkin, J, Stoesser, N, Karpe, F, Neville, M, Ploeg, R, Oliveira, M, Roberts, DJ, Lamikanra, AA, Tsang, HP, Bown, A, Vipond, R, Mentzer, AJ, Knight, JC, Kwok, AJ, Screaton, GR, Mongkolsapaya, J, Dejnirattisai, W, Supasa, P, Klenerman, P, Dold, C, Baillie, JK, Moore, SC, Openshaw, PJM, Semple, MG, Turtle, LCW, Ainsworth, M, Allcock, A, Beer, S, Bibi, S, Skelly, D, Stafford, L, Jeffrey, K, O’Donnell, D, Clutterbuck, E, Espinosa, A, Mendoza, M, Georgiou, D, Lockett, T, Martinez, J, Perez, E, Gallardo Sanchez, V, Scozzafava, G, Sobrinodiaz, A, Thraves, H & Joly, E 2021, 'A haemagglutination test for rapid detection of antibodies to SARS-CoV-2', Nature Communications, vol. 12, no. 1, 1951. https://doi.org/10.1038/s41467-021-22045-y

TY - JOUR

T1 - A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

AU - Townsend, Alain

AU - Rijal, Pramila

AU - Xiao, Julie

AU - Tan, Tiong Kit

AU - Huang, Kuan Ying A.

AU - Schimanski, Lisa

AU - Huo, Jiandong

AU - Gupta, Nimesh

AU - Rahikainen, Rolle

AU - Matthews, Philippa C.

AU - Crook, Derrick

AU - Hoosdally, Sarah

AU - Dunachie, Susanna

AU - Barnes, Eleanor

AU - Street, Teresa

AU - Conlon, Christopher P.

AU - Frater, John

AU - Arancibia-Cárcamo, Carolina V.

AU - Rudkin, Justine

AU - Stoesser, Nicole

AU - Karpe, Fredrik

AU - Neville, Matthew

AU - Ploeg, Rutger

AU - Oliveira, Marta

AU - Roberts, David J.

AU - Lamikanra, Abigail A.

AU - Tsang, Hoi Pat

AU - Bown, Abbie

AU - Vipond, Richard

AU - Mentzer, Alexander J.

AU - Knight, Julian C.

AU - Kwok, Andrew J.

AU - Screaton, Gavin R.

AU - Mongkolsapaya, Juthathip

AU - Dejnirattisai, Wanwisa

AU - Supasa, Piyada

AU - Klenerman, Paul

AU - Dold, Christina

AU - Baillie, J. Kenneth

AU - Moore, Shona C.

AU - Openshaw, Peter J.M.

AU - Semple, Malcolm G.

AU - Turtle, Lance C.W.

AU - Ainsworth, Mark

AU - Allcock, Alice

AU - Beer, Sally

AU - Bibi, Sagida

AU - Skelly, Donal

AU - Stafford, Lizzy

AU - Jeffrey, Katie

AU - O’Donnell, Denise

AU - Clutterbuck, Elizabeth

AU - Espinosa, Alexis

AU - Mendoza, Maria

AU - Georgiou, Dominique

AU - Lockett, Teresa

AU - Martinez, Jose

AU - Perez, Elena

AU - Gallardo Sanchez, Veronica

AU - Scozzafava, Giuseppe

AU - Sobrinodiaz, Alberto

AU - Thraves, Hannah

AU - Joly, Etienne

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.

AB - Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.

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U2 - 10.1038/s41467-021-22045-y

DO - 10.1038/s41467-021-22045-y

M3 - 文章

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AN - SCOPUS:85103541293

SN - 2041-1723

VL - 12

JO - Nature Communications

JF - Nature Communications

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ER -

A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

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