A medical innovation: a new and improved method of DNA extraction with electrowetting-on-dielectric of genetic testing in-vitro fertilization (IVF)

Cheng En Chiang, Hong Yuan Huang, Kai Ti Lin, Anand Baby Alias, Pei Jhen Lu, Yi Wen Wang, Tzu Hui Wu, Pei Shin Jiang, Chien An Chen, Da Jeng Yao*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

10 Scopus citations

Abstract

The current technique for the detection of fetus genotype by in-vitro fertilization (IVF) is quite expensive and is unsuitable/ inconvenient for all patients and even affects the embryonic growth cycle. We thus propose a new method to extract small amount of cell-free DNA (cf-DNA) from embryo culture medium. We introduce a new format for DNA extraction depending on digital microfluidics (DMF), electrowetting-on-dielectric (EWOD) and magnetic forces to separate, suspend DNA-coated magnetic particles. Nano-droplets (of nano-liter size) are electrostatically controlled on an array of insulated electrodes by EWOD system. By applying voltage to the various electrodes, multiple nano-droplets can be manipulated precisely in the same device. This feature enables one to extract DNA at ultra-small concentrations (fg/μL to pg/μL) from an embryo culture medium with an EWOD system. We applied our EWOD platform to extract a small amount of cf-DNA from a clinical embryo culture medium of mouse. In the future, we will distinguish the DNA in the real embryo buffer according to the length of the telomere. This feature would be valuable for the selection of an embryo before an implantation when the gene information can be recognized.

Original languageEnglish
Article number55
JournalMicrofluidics and Nanofluidics
Volume24
Issue number7
DOIs
StatePublished - 01 07 2020

Bibliographical note

Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

  • Biomedical
  • DNA extraction
  • Digital microfluidics
  • Electrowetting-on-dielectric
  • Embryo culture medium
  • In-vitro fertilization

Fingerprint

Dive into the research topics of 'A medical innovation: a new and improved method of DNA extraction with electrowetting-on-dielectric of genetic testing in-vitro fertilization (IVF)'. Together they form a unique fingerprint.

Cite this