Abstract
In this work, a new membrane-type microfluidic device was developed and incorporated with vancomycin-coated magnetic nanobeads for rapid bacteria detection, which only took 10 min to separate target bacteria from clinical human joint fluid (HJF) samples. Experimental results showed that the limit of detection could be significantly improved to be about 80 CFU/mL (or 13 CFU/reaction) by using a new three-dimensional membrane-type micromixer. As a result, we successfully demonstrated that the distinguishable fluorescence signal could be detected from a positive clinical HJF sample by using the developed diagnosis assay. The entire process including bacteria isolation in HJF, bacteria lysis, polymerase chain reaction and optical detection could be performed on a microfluidic system within 90 minutes.
Original language | English |
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Title of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
Publisher | Chemical and Biological Microsystems Society |
Pages | 1746-1749 |
Number of pages | 4 |
ISBN (Electronic) | 9781510897571 |
State | Published - 2018 |
Externally published | Yes |
Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan Duration: 11 11 2018 → 15 11 2018 |
Publication series
Name | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
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Volume | 3 |
Conference
Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
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Country/Territory | Taiwan |
City | Kaohsiung |
Period | 11/11/18 → 15/11/18 |
Bibliographical note
Publisher Copyright:Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
Keywords
- Bacteria detection
- Human joint fluid
- Micromixer
- Vancomycin