A membrane-type microfluidic device for rapid bacteria detection in clinical samples

Wen Bin Lee, Ting Hang Liu, Yi Sin Chen, Huey Ling You, Mel S. Lee*, Gwo Bin Lee

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this work, a new membrane-type microfluidic device was developed and incorporated with vancomycin-coated magnetic nanobeads for rapid bacteria detection, which only took 10 min to separate target bacteria from clinical human joint fluid (HJF) samples. Experimental results showed that the limit of detection could be significantly improved to be about 80 CFU/mL (or 13 CFU/reaction) by using a new three-dimensional membrane-type micromixer. As a result, we successfully demonstrated that the distinguishable fluorescence signal could be detected from a positive clinical HJF sample by using the developed diagnosis assay. The entire process including bacteria isolation in HJF, bacteria lysis, polymerase chain reaction and optical detection could be performed on a microfluidic system within 90 minutes.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1746-1749
Number of pages4
ISBN (Electronic)9781510897571
StatePublished - 2018
Externally publishedYes
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: 11 11 201815 11 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume3

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan
CityKaohsiung
Period11/11/1815/11/18

Bibliographical note

Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

Keywords

  • Bacteria detection
  • Human joint fluid
  • Micromixer
  • Vancomycin

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