A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity

Yang Zhao; Deyong Chen; Yana Luo; Hao Li; Bin Deng; Song Bin Huang; Tzu Keng Chiu; Min Hsien Wu; Rong Long; Junbo Wang; Jian Chen

doi:10.1109/Transducers.2013.6626999

A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity

Yang Zhao, Deyong Chen, Yana Luo, Hao Li, Bin Deng, Song Bin Huang, Tzu Keng Chiu, Min Hsien Wu, Rong Long, Junbo Wang, Jian Chen

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This paper presents a microfluidic system enabling continuous characterization of specific membrane capacitance (C _{specific membrane}) and cytoplasm conductivity (σ _cytoplasm) of single cells by aspirating cells continuously through a constriction channel with cell elongations and impedance profiles at two frequencies (1 kHz and 100 kHz) measured simultaneously. 1 kHz impedance data were used to evaluate cellular sealing properties and 100 kHz impedance data were used to quantify membrane capacitance and cytoplasm conductance, which were than translated to C_{specific membrane} and σ _cytoplasm based on the proposed equivalent circuit model. Four cell lines were used to evaluate this technique, producing C _{specific membrane} and σ_cytoplasm of 3.81±0.90 μF/cm² and 0.49±0.07 S/m (CRL-5803, n489), 4.28±1.05 μF/cm² and 0.45±0.08 S/m (CCL-185, n487), 3.67±1.00 μF/cm² and 0.47±0.09 S/m (CRL-1932, n302), as well as 4.53±1.51 μF/cm² and 0.55±0.14 S/m (CRL-1999, n216), respectively.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII
Subtitle of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages	1239-1242
Number of pages	4
DOIs	https://doi.org/10.1109/Transducers.2013.6626999
State	Published - 2013
Event	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain Duration: 16 06 2013 → 20 06 2013

Publication series

Name	2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Conference

Conference	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/Territory	Spain
City	Barcelona
Period	16/06/13 → 20/06/13

Keywords

Cytoplasm Conductivity
Microfluidics
Single-Cell Analysis
Specific Membrane Capacitance

Access to Document

10.1109/Transducers.2013.6626999

Cite this

Zhao, Y., Chen, D., Luo, Y., Li, H., Deng, B., Huang, S. B., Chiu, T. K., Wu, M. H., Long, R., Wang, J., & Chen, J. (2013). A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 1239-1242). Article 6626999 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). https://doi.org/10.1109/Transducers.2013.6626999

Zhao, Yang ; Chen, Deyong ; Luo, Yana et al. / A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity. 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. pp. 1239-1242 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

@inproceedings{1c56909349334c8eb256471e7ba93227,

title = "A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity",

abstract = "This paper presents a microfluidic system enabling continuous characterization of specific membrane capacitance (C specific membrane) and cytoplasm conductivity (σ cytoplasm) of single cells by aspirating cells continuously through a constriction channel with cell elongations and impedance profiles at two frequencies (1 kHz and 100 kHz) measured simultaneously. 1 kHz impedance data were used to evaluate cellular sealing properties and 100 kHz impedance data were used to quantify membrane capacitance and cytoplasm conductance, which were than translated to Cspecific membrane and σ cytoplasm based on the proposed equivalent circuit model. Four cell lines were used to evaluate this technique, producing C specific membrane and σcytoplasm of 3.81±0.90 μF/cm2 and 0.49±0.07 S/m (CRL-5803, n489), 4.28±1.05 μF/cm2 and 0.45±0.08 S/m (CCL-185, n487), 3.67±1.00 μF/cm2 and 0.47±0.09 S/m (CRL-1932, n302), as well as 4.53±1.51 μF/cm2 and 0.55±0.14 S/m (CRL-1999, n216), respectively.",

keywords = "Cytoplasm Conductivity, Microfluidics, Single-Cell Analysis, Specific Membrane Capacitance",

author = "Yang Zhao and Deyong Chen and Yana Luo and Hao Li and Bin Deng and Huang, {Song Bin} and Chiu, {Tzu Keng} and Wu, {Min Hsien} and Rong Long and Junbo Wang and Jian Chen",

year = "2013",

doi = "10.1109/Transducers.2013.6626999",

language = "英语",

isbn = "9781467359818",

series = "2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013",

pages = "1239--1242",

booktitle = "2013 Transducers and Eurosensors XXVII",

note = "2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 ; Conference date: 16-06-2013 Through 20-06-2013",

}

Zhao, Y, Chen, D, Luo, Y, Li, H, Deng, B, Huang, SB, Chiu, TK, Wu, MH, Long, R, Wang, J & Chen, J 2013, A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6626999, 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, pp. 1239-1242, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 16/06/13. https://doi.org/10.1109/Transducers.2013.6626999

A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity. / Zhao, Yang; Chen, Deyong; Luo, Yana et al.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 1239-1242 6626999 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity

AU - Zhao, Yang

AU - Chen, Deyong

AU - Luo, Yana

AU - Li, Hao

AU - Deng, Bin

AU - Huang, Song Bin

AU - Chiu, Tzu Keng

AU - Wu, Min Hsien

AU - Long, Rong

AU - Wang, Junbo

AU - Chen, Jian

PY - 2013

Y1 - 2013

N2 - This paper presents a microfluidic system enabling continuous characterization of specific membrane capacitance (C specific membrane) and cytoplasm conductivity (σ cytoplasm) of single cells by aspirating cells continuously through a constriction channel with cell elongations and impedance profiles at two frequencies (1 kHz and 100 kHz) measured simultaneously. 1 kHz impedance data were used to evaluate cellular sealing properties and 100 kHz impedance data were used to quantify membrane capacitance and cytoplasm conductance, which were than translated to Cspecific membrane and σ cytoplasm based on the proposed equivalent circuit model. Four cell lines were used to evaluate this technique, producing C specific membrane and σcytoplasm of 3.81±0.90 μF/cm2 and 0.49±0.07 S/m (CRL-5803, n489), 4.28±1.05 μF/cm2 and 0.45±0.08 S/m (CCL-185, n487), 3.67±1.00 μF/cm2 and 0.47±0.09 S/m (CRL-1932, n302), as well as 4.53±1.51 μF/cm2 and 0.55±0.14 S/m (CRL-1999, n216), respectively.

AB - This paper presents a microfluidic system enabling continuous characterization of specific membrane capacitance (C specific membrane) and cytoplasm conductivity (σ cytoplasm) of single cells by aspirating cells continuously through a constriction channel with cell elongations and impedance profiles at two frequencies (1 kHz and 100 kHz) measured simultaneously. 1 kHz impedance data were used to evaluate cellular sealing properties and 100 kHz impedance data were used to quantify membrane capacitance and cytoplasm conductance, which were than translated to Cspecific membrane and σ cytoplasm based on the proposed equivalent circuit model. Four cell lines were used to evaluate this technique, producing C specific membrane and σcytoplasm of 3.81±0.90 μF/cm2 and 0.49±0.07 S/m (CRL-5803, n489), 4.28±1.05 μF/cm2 and 0.45±0.08 S/m (CCL-185, n487), 3.67±1.00 μF/cm2 and 0.47±0.09 S/m (CRL-1932, n302), as well as 4.53±1.51 μF/cm2 and 0.55±0.14 S/m (CRL-1999, n216), respectively.

KW - Cytoplasm Conductivity

KW - Microfluidics

KW - Single-Cell Analysis

KW - Specific Membrane Capacitance

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U2 - 10.1109/Transducers.2013.6626999

DO - 10.1109/Transducers.2013.6626999

M3 - 会议稿件

AN - SCOPUS:84891691841

SN - 9781467359818

T3 - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

SP - 1239

EP - 1242

BT - 2013 Transducers and Eurosensors XXVII

T2 - 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Y2 - 16 June 2013 through 20 June 2013

ER -

Zhao Y, Chen D, Luo Y, Li H, Deng B, Huang SB et al. A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 1239-1242. 6626999. (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). doi: 10.1109/Transducers.2013.6626999

A microfluidic device capable of continuous quantification of single-cell specific membrane capacitance and cytoplasm conductivity

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Keywords

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