A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method

Wen Bin Lee; Wen Hsin Chang; Huey Ling You; Mel S. Lee; Gwo Bin Lee

doi:10.1109/MEMSYS.2017.7863480

A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method

Wen Bin Lee, Wen Hsin Chang, Huey Ling You, Mel S. Lee, Gwo Bin Lee^*

^*Corresponding author for this work

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

A simple microfluidic device which can perform antimicrobial susceptibility testing (AST) of combined antibiotics against antibiotics-resistant bacteria has been presented. A new approach to determine the fractional inhibitory concentration (FIC) index of two antibiotics by performing broth dilutions automatically was proposed and its performance was highly consistent with manual operation. The experimental results showed that the developed device could achieve on-chip dispensation/dilution/mixing accurately and interpret AST results visually. A test using two common antibiotics (vancomycin and ceftazidime) was performed on one clinical isolate of vancomycin-intermediate Staphylococcus aureus (VISA) based on the FIC indices. It is the first time that a microfluidic device was demonstrated to be capable of determining the FIC index of combined antibiotics against bacteria by performing AST automatically. With its high flexibility and reliability, the proposed device provides a simple method to explore drug cocktails for combined antibiotic therapy.

Original language	English
Title of host publication	2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	605-607
Number of pages	3
ISBN (Electronic)	9781509050789
DOIs	https://doi.org/10.1109/MEMSYS.2017.7863480
State	Published - 23 02 2017
Event	30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 - Las Vegas, United States Duration: 22 01 2017 → 26 01 2017

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
Country/Territory	United States
City	Las Vegas
Period	22/01/17 → 26/01/17

Bibliographical note

Publisher Copyright:
© 2017 IEEE.

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10.1109/MEMSYS.2017.7863480

Cite this

Lee, W. B., Chang, W. H., You, H. L., Lee, M. S., & Lee, G. B. (2017). A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 (pp. 605-607). Article 7863480 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2017.7863480

Lee, Wen Bin ; Chang, Wen Hsin ; You, Huey Ling et al. / A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method. 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 605-607 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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title = "A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method",

abstract = "A simple microfluidic device which can perform antimicrobial susceptibility testing (AST) of combined antibiotics against antibiotics-resistant bacteria has been presented. A new approach to determine the fractional inhibitory concentration (FIC) index of two antibiotics by performing broth dilutions automatically was proposed and its performance was highly consistent with manual operation. The experimental results showed that the developed device could achieve on-chip dispensation/dilution/mixing accurately and interpret AST results visually. A test using two common antibiotics (vancomycin and ceftazidime) was performed on one clinical isolate of vancomycin-intermediate Staphylococcus aureus (VISA) based on the FIC indices. It is the first time that a microfluidic device was demonstrated to be capable of determining the FIC index of combined antibiotics against bacteria by performing AST automatically. With its high flexibility and reliability, the proposed device provides a simple method to explore drug cocktails for combined antibiotic therapy.",

author = "Lee, {Wen Bin} and Chang, {Wen Hsin} and You, {Huey Ling} and Lee, {Mel S.} and Lee, {Gwo Bin}",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 ; Conference date: 22-01-2017 Through 26-01-2017",

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Lee, WB, Chang, WH, You, HL, Lee, MS & Lee, GB 2017, A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method. in 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017., 7863480, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 605-607, 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017, Las Vegas, United States, 22/01/17. https://doi.org/10.1109/MEMSYS.2017.7863480

A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method. / Lee, Wen Bin; Chang, Wen Hsin; You, Huey Ling et al.
2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 605-607 7863480 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Lee WB, Chang WH, You HL, Lee MS, Lee GB. A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 605-607. 7863480. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2017.7863480

A microfluidic device for antimicrobial susceptibility testing of combined antibiotics by using broth dilution method

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