A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro

Chi Fan Chen; Kuo Wei Chang; Ting Ru Yueh; Hong Yuan Huang; Chen Shien Liu

doi:10.1109/NEMS.2014.6908808

A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro

Chi Fan Chen^*
, Kuo Wei Chang
, Ting Ru Yueh
, Hong Yuan Huang
, Chen Shien Liu

^*Corresponding author for this work

National Tsing Hua University
Chang Gung Memorial Hospital

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Nowadays, infertility is one of the major problems for many of married couples. Thus, there are a wide range of researches on infertility where the in vitro fertilization is the best known. However, the conventional approach to fertilize embryo requires more on manual operation times that may causes some damage of embryos. To overcome the drawback of conventional method, we present a microfluidic chip to trap cells and mimic a uterus in vitro by co-culturing stromal cells and mouse embryos. By dynamic resistance design, mouse embryos can be trapped individually. In this way, we can grow them by co-culturing with stromal cells in an open system with constant medium supply, known as dynamic perfusion. The novel method can reduce the manual operation and the possibility of damage to embryos. Also, the chip can track and manage individual embryos easily.

Original language	English
Title of host publication	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	281-284
Number of pages	4
ISBN (Electronic)	9781479947270
DOIs	https://doi.org/10.1109/NEMS.2014.6908808
State	Published - 23 09 2014
Externally published	Yes
Event	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 - Waikiki Beach, United States Duration: 13 04 2014 → 16 04 2014

Publication series

Name	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

Conference

Conference	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
Country/Territory	United States
City	Waikiki Beach
Period	13/04/14 → 16/04/14

Bibliographical note

Publisher Copyright:
© 2014 IEEE.

Keywords

cell trapping
co-culture
embryo
in vitro fertilization

Access to Document

10.1109/NEMS.2014.6908808

Cite this

Chen, C. F., Chang, K. W., Yueh, T. R., Huang, H. Y., & Liu, C. S. (2014). A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro. In 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 (pp. 281-284). Article 6908808 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2014.6908808

Chen, Chi Fan ; Chang, Kuo Wei ; Yueh, Ting Ru et al. / A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro. 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 281-284 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014).

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title = "A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro",

abstract = "Nowadays, infertility is one of the major problems for many of married couples. Thus, there are a wide range of researches on infertility where the in vitro fertilization is the best known. However, the conventional approach to fertilize embryo requires more on manual operation times that may causes some damage of embryos. To overcome the drawback of conventional method, we present a microfluidic chip to trap cells and mimic a uterus in vitro by co-culturing stromal cells and mouse embryos. By dynamic resistance design, mouse embryos can be trapped individually. In this way, we can grow them by co-culturing with stromal cells in an open system with constant medium supply, known as dynamic perfusion. The novel method can reduce the manual operation and the possibility of damage to embryos. Also, the chip can track and manage individual embryos easily.",

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author = "Chen, \{Chi Fan\} and Chang, \{Kuo Wei\} and Yueh, \{Ting Ru\} and Huang, \{Hong Yuan\} and Liu, \{Chen Shien\}",

note = "Publisher Copyright: {\textcopyright} 2014 IEEE.; 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 ; Conference date: 13-04-2014 Through 16-04-2014",

year = "2014",

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doi = "10.1109/NEMS.2014.6908808",

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Chen, CF, Chang, KW, Yueh, TR, Huang, HY & Liu, CS 2014, A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro. in 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014., 6908808, 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014, Institute of Electrical and Electronics Engineers Inc., pp. 281-284, 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014, Waikiki Beach, United States, 13/04/14. https://doi.org/10.1109/NEMS.2014.6908808

A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro. / Chen, Chi Fan; Chang, Kuo Wei; Yueh, Ting Ru et al.
9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. p. 281-284 6908808 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro

AU - Chen, Chi Fan

AU - Chang, Kuo Wei

AU - Yueh, Ting Ru

AU - Huang, Hong Yuan

AU - Liu, Chen Shien

PY - 2014/9/23

Y1 - 2014/9/23

N2 - Nowadays, infertility is one of the major problems for many of married couples. Thus, there are a wide range of researches on infertility where the in vitro fertilization is the best known. However, the conventional approach to fertilize embryo requires more on manual operation times that may causes some damage of embryos. To overcome the drawback of conventional method, we present a microfluidic chip to trap cells and mimic a uterus in vitro by co-culturing stromal cells and mouse embryos. By dynamic resistance design, mouse embryos can be trapped individually. In this way, we can grow them by co-culturing with stromal cells in an open system with constant medium supply, known as dynamic perfusion. The novel method can reduce the manual operation and the possibility of damage to embryos. Also, the chip can track and manage individual embryos easily.

AB - Nowadays, infertility is one of the major problems for many of married couples. Thus, there are a wide range of researches on infertility where the in vitro fertilization is the best known. However, the conventional approach to fertilize embryo requires more on manual operation times that may causes some damage of embryos. To overcome the drawback of conventional method, we present a microfluidic chip to trap cells and mimic a uterus in vitro by co-culturing stromal cells and mouse embryos. By dynamic resistance design, mouse embryos can be trapped individually. In this way, we can grow them by co-culturing with stromal cells in an open system with constant medium supply, known as dynamic perfusion. The novel method can reduce the manual operation and the possibility of damage to embryos. Also, the chip can track and manage individual embryos easily.

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KW - co-culture

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KW - in vitro fertilization

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BT - 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

PB - Institute of Electrical and Electronics Engineers Inc.

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Y2 - 13 April 2014 through 16 April 2014

ER -

Chen CF, Chang KW, Yueh TR, Huang HY, Liu CS. A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro. In 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc. 2014. p. 281-284. 6908808. (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014). doi: 10.1109/NEMS.2014.6908808

A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro

Abstract

Publication series

Conference

Bibliographical note

Keywords

Access to Document

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