A microfluidic device with automatic embryos trapping and co-culture with human stromal cells

Tzu Wei Lo; Yi Jung Sung; Shalaka S. Bhosale; Yi Wen Wang; Hong Yuan Huang; Cheng Hsien Liu

doi:10.1109/TRANSDUCERS.2017.7994398

A microfluidic device with automatic embryos trapping and co-culture with human stromal cells

Tzu Wei Lo^*, Yi Jung Sung, Shalaka S. Bhosale, Yi Wen Wang, Hong Yuan Huang, Cheng Hsien Liu

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In order to overcome the limitations of the traditional In Vitro Fertilization (IVF) culture method, this microfluidic chip is designed to integrate multiple functions to achieve the purpose of the embryos culturing in vitro. The chip combines the mechanism of dynamic flow resistance to trap and locate the embryos and co-culture with the stromal cells secreting the growth factors to give a better environment for embryos. Dynamic culture can not only carry metabolic waste off but also supply fresh medium to maintain stable osmotic pressure and pH value. The idea is combining the concept of flow-resistant by analogy with electric resistance to design automatic trapping structure. The advantages are reducing damage to embryo because of artificial operation and convenient observation. Finally, the research succeeded in giving birth to the mice.

Original language	English
Title of host publication	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1718-1721
Number of pages	4
ISBN (Electronic)	9781538627310
DOIs	https://doi.org/10.1109/TRANSDUCERS.2017.7994398
State	Published - 26 07 2017
Externally published	Yes
Event	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan Duration: 18 06 2017 → 22 06 2017

Publication series

Name	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

Conference	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Country/Territory	Taiwan
City	Kaohsiung
Period	18/06/17 → 22/06/17

Bibliographical note

Publisher Copyright:
© 2017 IEEE.

Keywords

Reproductive medicine
dynamic perfusion
embryo co-culture
embryo trapping

Access to Document

10.1109/TRANSDUCERS.2017.7994398

Cite this

Lo, T. W., Sung, Y. J., Bhosale, S. S., Wang, Y. W., Huang, H. Y., & Liu, C. H. (2017). A microfluidic device with automatic embryos trapping and co-culture with human stromal cells. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 1718-1721). Article 7994398 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994398

Lo, Tzu Wei ; Sung, Yi Jung ; Bhosale, Shalaka S. et al. / A microfluidic device with automatic embryos trapping and co-culture with human stromal cells. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 1718-1721 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

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title = "A microfluidic device with automatic embryos trapping and co-culture with human stromal cells",

abstract = "In order to overcome the limitations of the traditional In Vitro Fertilization (IVF) culture method, this microfluidic chip is designed to integrate multiple functions to achieve the purpose of the embryos culturing in vitro. The chip combines the mechanism of dynamic flow resistance to trap and locate the embryos and co-culture with the stromal cells secreting the growth factors to give a better environment for embryos. Dynamic culture can not only carry metabolic waste off but also supply fresh medium to maintain stable osmotic pressure and pH value. The idea is combining the concept of flow-resistant by analogy with electric resistance to design automatic trapping structure. The advantages are reducing damage to embryo because of artificial operation and convenient observation. Finally, the research succeeded in giving birth to the mice.",

keywords = "Reproductive medicine, dynamic perfusion, embryo co-culture, embryo trapping",

author = "Lo, {Tzu Wei} and Sung, {Yi Jung} and Bhosale, {Shalaka S.} and Wang, {Yi Wen} and Huang, {Hong Yuan} and Liu, {Cheng Hsien}",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 ; Conference date: 18-06-2017 Through 22-06-2017",

year = "2017",

month = jul,

day = "26",

doi = "10.1109/TRANSDUCERS.2017.7994398",

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series = "TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems",

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Lo, TW, Sung, YJ, Bhosale, SS, Wang, YW, Huang, HY & Liu, CH 2017, A microfluidic device with automatic embryos trapping and co-culture with human stromal cells. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994398, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., pp. 1718-1721, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, 18/06/17. https://doi.org/10.1109/TRANSDUCERS.2017.7994398

A microfluidic device with automatic embryos trapping and co-culture with human stromal cells. / Lo, Tzu Wei; Sung, Yi Jung; Bhosale, Shalaka S. et al.
TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 1718-1721 7994398 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A microfluidic device with automatic embryos trapping and co-culture with human stromal cells

AU - Lo, Tzu Wei

AU - Sung, Yi Jung

AU - Bhosale, Shalaka S.

AU - Wang, Yi Wen

AU - Huang, Hong Yuan

AU - Liu, Cheng Hsien

PY - 2017/7/26

Y1 - 2017/7/26

N2 - In order to overcome the limitations of the traditional In Vitro Fertilization (IVF) culture method, this microfluidic chip is designed to integrate multiple functions to achieve the purpose of the embryos culturing in vitro. The chip combines the mechanism of dynamic flow resistance to trap and locate the embryos and co-culture with the stromal cells secreting the growth factors to give a better environment for embryos. Dynamic culture can not only carry metabolic waste off but also supply fresh medium to maintain stable osmotic pressure and pH value. The idea is combining the concept of flow-resistant by analogy with electric resistance to design automatic trapping structure. The advantages are reducing damage to embryo because of artificial operation and convenient observation. Finally, the research succeeded in giving birth to the mice.

AB - In order to overcome the limitations of the traditional In Vitro Fertilization (IVF) culture method, this microfluidic chip is designed to integrate multiple functions to achieve the purpose of the embryos culturing in vitro. The chip combines the mechanism of dynamic flow resistance to trap and locate the embryos and co-culture with the stromal cells secreting the growth factors to give a better environment for embryos. Dynamic culture can not only carry metabolic waste off but also supply fresh medium to maintain stable osmotic pressure and pH value. The idea is combining the concept of flow-resistant by analogy with electric resistance to design automatic trapping structure. The advantages are reducing damage to embryo because of artificial operation and convenient observation. Finally, the research succeeded in giving birth to the mice.

KW - Reproductive medicine

KW - dynamic perfusion

KW - embryo co-culture

KW - embryo trapping

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BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

PB - Institute of Electrical and Electronics Engineers Inc.

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Y2 - 18 June 2017 through 22 June 2017

ER -

Lo TW, Sung YJ, Bhosale SS, Wang YW, Huang HY, Liu CH. A microfluidic device with automatic embryos trapping and co-culture with human stromal cells. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 1718-1721. 7994398. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/TRANSDUCERS.2017.7994398

A microfluidic device with automatic embryos trapping and co-culture with human stromal cells

Abstract

Publication series

Conference

Bibliographical note

Keywords

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