A microfluidic platform for detection and quantification of two biomarkers for rheumatoid arthritis

Tsung Hsien Wu; Yi Cheng Tsai; Feng Chih Kuo; Mel S. Lee; Chih Chien Hu; Gwo Bin Lee

doi:10.1016/j.snb.2023.133587

A microfluidic platform for detection and quantification of two biomarkers for rheumatoid arthritis

Tsung Hsien Wu, Yi Cheng Tsai, Feng Chih Kuo, Mel S. Lee, Chih Chien Hu^*, Gwo Bin Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

20 Scopus citations

Abstract

Early detection of rheumatoid factors (RF) and cyclic citrullinated peptide antibodies (anti-CCP) has been proven to be effective in diagnosing, and even preventing progression to more advanced-stages, of rheumatoid arthritis (RA), thereby decreasing irreversible damage to joints. Herein a novel, dual-target detection approach was integrated in a 45 mm × 62 mm microfluidic chip that featured a symmetrical design in which magnetic beads surface-coated with fragment-crystallizable regions of immunoglobulin G and biotinylated CCP enabled detection of these two RA biomarkers within 55 min with only 5 μL of serum. This device could therefore be useful for rapid clinical diagnosis of this all-too-common ailment.

Original language	English
Article number	133587
Journal	Sensors and Actuators, B: Chemical
Volume	383
DOIs	https://doi.org/10.1016/j.snb.2023.133587
State	Published - 15 05 2023
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2023 Elsevier B.V.

Keywords

Anti-cyclic citrullinated peptide
Enzyme-linked immunosorbent assay (ELISA)
Microfluidics
Rheumatoid arthritis
Rheumatoid factor

Access to Document

10.1016/j.snb.2023.133587

Cite this

@article{883353c4c891455fb18fb0586979189f,

title = "A microfluidic platform for detection and quantification of two biomarkers for rheumatoid arthritis",

abstract = "Early detection of rheumatoid factors (RF) and cyclic citrullinated peptide antibodies (anti-CCP) has been proven to be effective in diagnosing, and even preventing progression to more advanced-stages, of rheumatoid arthritis (RA), thereby decreasing irreversible damage to joints. Herein a novel, dual-target detection approach was integrated in a 45 mm × 62 mm microfluidic chip that featured a symmetrical design in which magnetic beads surface-coated with fragment-crystallizable regions of immunoglobulin G and biotinylated CCP enabled detection of these two RA biomarkers within 55 min with only 5 μL of serum. This device could therefore be useful for rapid clinical diagnosis of this all-too-common ailment.",

keywords = "Anti-cyclic citrullinated peptide, Enzyme-linked immunosorbent assay (ELISA), Microfluidics, Rheumatoid arthritis, Rheumatoid factor",

author = "Wu, \{Tsung Hsien\} and Tsai, \{Yi Cheng\} and Kuo, \{Feng Chih\} and Lee, \{Mel S.\} and Hu, \{Chih Chien\} and Lee, \{Gwo Bin\}",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier B.V.",

year = "2023",

month = may,

day = "15",

doi = "10.1016/j.snb.2023.133587",

language = "英语",

volume = "383",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - A microfluidic platform for detection and quantification of two biomarkers for rheumatoid arthritis

AU - Wu, Tsung Hsien

AU - Tsai, Yi Cheng

AU - Kuo, Feng Chih

AU - Lee, Mel S.

AU - Hu, Chih Chien

AU - Lee, Gwo Bin

PY - 2023/5/15

Y1 - 2023/5/15

N2 - Early detection of rheumatoid factors (RF) and cyclic citrullinated peptide antibodies (anti-CCP) has been proven to be effective in diagnosing, and even preventing progression to more advanced-stages, of rheumatoid arthritis (RA), thereby decreasing irreversible damage to joints. Herein a novel, dual-target detection approach was integrated in a 45 mm × 62 mm microfluidic chip that featured a symmetrical design in which magnetic beads surface-coated with fragment-crystallizable regions of immunoglobulin G and biotinylated CCP enabled detection of these two RA biomarkers within 55 min with only 5 μL of serum. This device could therefore be useful for rapid clinical diagnosis of this all-too-common ailment.

AB - Early detection of rheumatoid factors (RF) and cyclic citrullinated peptide antibodies (anti-CCP) has been proven to be effective in diagnosing, and even preventing progression to more advanced-stages, of rheumatoid arthritis (RA), thereby decreasing irreversible damage to joints. Herein a novel, dual-target detection approach was integrated in a 45 mm × 62 mm microfluidic chip that featured a symmetrical design in which magnetic beads surface-coated with fragment-crystallizable regions of immunoglobulin G and biotinylated CCP enabled detection of these two RA biomarkers within 55 min with only 5 μL of serum. This device could therefore be useful for rapid clinical diagnosis of this all-too-common ailment.

KW - Anti-cyclic citrullinated peptide

KW - Enzyme-linked immunosorbent assay (ELISA)

KW - Microfluidics

KW - Rheumatoid arthritis

KW - Rheumatoid factor

UR - https://www.scopus.com/pages/publications/85149338475

U2 - 10.1016/j.snb.2023.133587

DO - 10.1016/j.snb.2023.133587

M3 - 文章

AN - SCOPUS:85149338475

SN - 0925-4005

VL - 383

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

M1 - 133587

ER -

A microfluidic platform for detection and quantification of two biomarkers for rheumatoid arthritis

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this