A microfluidic system enabling continuous characterization of single-cell specific membrane capacitance and cytoplasm conductivity

Yang Zhao, Deyong Chen, Hao Li, Yana Luo, Bin Deng, Song Bin Huang, Tzu Keng Chiu, Min Hsien Wu, Rong Long, Junbo Wang*, Jian Chen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

This paper presents a microfluidic system enabling continuous characterization of specific membrane capacitance (C specific membrane) and cytoplasm conductivity (σ cytoplasm) of single biological cells. In this study, cells were aspirated through a constriction channel while cell elongations and impedance profiles at 1 kHz and 100 kHz were measured simultaneously using microscopy imaging and a lock-in amplifier. Based on the proposed equivalent circuit model, raw data were translated to Cspecific membrane and σcytoplasm, which were 3.67±1.00 vs. 4.53±1.51 μF/cm2 and 0.47±0.09 vs. 0.55±0.14 S/m for the kidney tumor cell line of 786-O (n=302) and the vascular smooth muscle cell line of T2 (n=216), respectively.

Original languageEnglish
Title of host publication8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Pages344-347
Number of pages4
DOIs
StatePublished - 2013
Event8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 - Suzhou, China
Duration: 07 04 201310 04 2013

Publication series

Name8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013

Conference

Conference8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Country/TerritoryChina
CitySuzhou
Period07/04/1310/04/13

Keywords

  • Cytoplasm Conductivity
  • Microfluidics
  • Single-Cell Analysis
  • Specific Membrane Capacitance

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