A Multifunctional Microfluidic Device for Blood Typing and Primary Screening of Blood Diseases

Jia Hui Lin, Tsung Ting Tsai, Qiang Zeng, Chun Yen Chang, Jun Yu Guo, Chi Jui Lin, Chien Fu Chen*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

18 Scopus citations

Abstract

In this work, we demonstrate a multifunctional, portable, and disposable microfluidic device for blood typing and primary screening of blood diseases. Preloaded antibodies (anti-A, anti-B, and anti-D) interact with injected whole blood cells to cause an agglutination reaction that blocks a microslit in the microfluidic channel to accumulate red blood cells and form a visible red line that can be easily read to determine the blood type. Moreover, the different blood density and agglutination properties of normal and subtype blood groups, as well as different blood diseases, including anemia and polycythemia vera, generate different lengths of blood agglutination within the channels, which allows us to successfully screen these various conditions in as little as 2 min. The required blood volume for each test is just 1 μL, which can be obtained by minimally invasive finger pricking. This novel method of observing agglutinated red blood cells to distinguish blood types and diseases is both feasible and affordable, suggesting its promise for use in areas with limited resources.

Original languageEnglish
Pages (from-to)3082-3090
Number of pages9
JournalACS Sensors
Volume5
Issue number10
DOIs
StatePublished - 23 10 2020

Bibliographical note

Publisher Copyright:
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Keywords

  • anemia
  • blood agglutination test
  • blood typing
  • point-of-care diagnosis
  • polycythemia

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