A new human hepatoma cell line: establishment and characterization

Y. M. Lin; C. P. Hu; C. K. Chou; T. W. O-Lee; K. T. Wuu; T. Y. Chen; F. K. Peng; T. J. Liu; J. L. Ko; C. M. Chang

A new human hepatoma cell line: establishment and characterization

Y. M. Lin^*, C. P. Hu, C. K. Chou, T. W. O-Lee, K. T. Wuu, T. Y. Chen, F. K. Peng, T. J. Liu, J. L. Ko, C. M. Chang

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

17 Scopus citations

Abstract

We have grown a human hepatoma cell line, designated as HA22T/VGH, from a 52-yr-old male hepatoma patient since July 1, 1980. This cell line has been subcultured more than 100 passages. The chromosome analysis of HA22T/VGH indicated that the chromosome numbers varied from 70 to 146, with the mode of 73. Methylcellulose soft agar assay showed that approximately 40% of the HA22T/VGH cells formed colonies. The HA22T/VGH produced tumors in nude mice. Histopathological studies of the tumor revealed the arrangement of hepatoma. Detected by the complement fixation method HA22T/VGH cells secreted ceruloplasmin, Factor B, C3, C4, Gc-globulin and alpha 1-acid-glycoprotein. These cells contained the liver associated enzymes: alanine amino transferase, tyrosine amino transferase and gamma-glutamyl transferase. HBsAg and alpha-fetoprotein were not detectable in the HA22T/VGH culture media or cell lysates by the radioimmunoassay.

Original language	English
Pages (from-to)	193-201
Number of pages	9
Journal	Chinese Journal of Microbiology and Immunology
Volume	15
Issue number	3
State	Published - 08 1982
Externally published	Yes

Cite this

@article{fa0b49facc6145d79704fda45ee279ed,

title = "A new human hepatoma cell line: establishment and characterization",

abstract = "We have grown a human hepatoma cell line, designated as HA22T/VGH, from a 52-yr-old male hepatoma patient since July 1, 1980. This cell line has been subcultured more than 100 passages. The chromosome analysis of HA22T/VGH indicated that the chromosome numbers varied from 70 to 146, with the mode of 73. Methylcellulose soft agar assay showed that approximately 40% of the HA22T/VGH cells formed colonies. The HA22T/VGH produced tumors in nude mice. Histopathological studies of the tumor revealed the arrangement of hepatoma. Detected by the complement fixation method HA22T/VGH cells secreted ceruloplasmin, Factor B, C3, C4, Gc-globulin and alpha 1-acid-glycoprotein. These cells contained the liver associated enzymes: alanine amino transferase, tyrosine amino transferase and gamma-glutamyl transferase. HBsAg and alpha-fetoprotein were not detectable in the HA22T/VGH culture media or cell lysates by the radioimmunoassay.",

author = "Lin, {Y. M.} and Hu, {C. P.} and Chou, {C. K.} and O-Lee, {T. W.} and Wuu, {K. T.} and Chen, {T. Y.} and Peng, {F. K.} and Liu, {T. J.} and Ko, {J. L.} and Chang, {C. M.}",

year = "1982",

month = aug,

language = "英语",

volume = "15",

pages = "193--201",

journal = "Chinese Journal of Microbiology and Immunology",

issn = "0253-2662",

publisher = "Lippincott Williams & Wilkins Asia",

number = "3",

}

TY - JOUR

T1 - A new human hepatoma cell line

T2 - establishment and characterization

AU - Lin, Y. M.

AU - Hu, C. P.

AU - Chou, C. K.

AU - O-Lee, T. W.

AU - Wuu, K. T.

AU - Chen, T. Y.

AU - Peng, F. K.

AU - Liu, T. J.

AU - Ko, J. L.

AU - Chang, C. M.

PY - 1982/8

Y1 - 1982/8

N2 - We have grown a human hepatoma cell line, designated as HA22T/VGH, from a 52-yr-old male hepatoma patient since July 1, 1980. This cell line has been subcultured more than 100 passages. The chromosome analysis of HA22T/VGH indicated that the chromosome numbers varied from 70 to 146, with the mode of 73. Methylcellulose soft agar assay showed that approximately 40% of the HA22T/VGH cells formed colonies. The HA22T/VGH produced tumors in nude mice. Histopathological studies of the tumor revealed the arrangement of hepatoma. Detected by the complement fixation method HA22T/VGH cells secreted ceruloplasmin, Factor B, C3, C4, Gc-globulin and alpha 1-acid-glycoprotein. These cells contained the liver associated enzymes: alanine amino transferase, tyrosine amino transferase and gamma-glutamyl transferase. HBsAg and alpha-fetoprotein were not detectable in the HA22T/VGH culture media or cell lysates by the radioimmunoassay.

AB - We have grown a human hepatoma cell line, designated as HA22T/VGH, from a 52-yr-old male hepatoma patient since July 1, 1980. This cell line has been subcultured more than 100 passages. The chromosome analysis of HA22T/VGH indicated that the chromosome numbers varied from 70 to 146, with the mode of 73. Methylcellulose soft agar assay showed that approximately 40% of the HA22T/VGH cells formed colonies. The HA22T/VGH produced tumors in nude mice. Histopathological studies of the tumor revealed the arrangement of hepatoma. Detected by the complement fixation method HA22T/VGH cells secreted ceruloplasmin, Factor B, C3, C4, Gc-globulin and alpha 1-acid-glycoprotein. These cells contained the liver associated enzymes: alanine amino transferase, tyrosine amino transferase and gamma-glutamyl transferase. HBsAg and alpha-fetoprotein were not detectable in the HA22T/VGH culture media or cell lysates by the radioimmunoassay.

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M3 - 文章

C2 - 6291875

AN - SCOPUS:0020170801

SN - 0253-2662

VL - 15

SP - 193

EP - 201

JO - Chinese Journal of Microbiology and Immunology

JF - Chinese Journal of Microbiology and Immunology

IS - 3

ER -

A new human hepatoma cell line: establishment and characterization

Abstract

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