A new platform for manipulating a single dna molecule by using opticallay-induced dielectrophoresis

Yen Heng Lin, Chen Min Chang, Gwo Bin Lee*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

This paper presents a new platform capable of manipulating a single DNA molecule based on optically-induced dielectrophoretic (ODEP) forces. The ends of λ-phage DNA were bound with polystyrene beads which can be manipulated by optical images generated by a digital projector. The manipulation process of fluorescent-labeled DNA was observed in real time under the ODEP operation. Two kinds of DNA manipulation were conducted including DNA stretching and revolving through the computer-controlled optical images. The stretching force was also measured to estimate the manipulation ability of DNA molecule in this platform. The maximum stretching force was estimated to be about 49.73 pN when binding with 10 μm polystyrene beads. The developed platform may be a promising tool for single DNA molecule investigation.

Original languageEnglish
Title of host publicationTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages1590-1593
Number of pages4
DOIs
StatePublished - 2009
Externally publishedYes
EventTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems - Denver, CO, United States
Duration: 21 06 200925 06 2009

Publication series

NameTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

Conference

ConferenceTRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Country/TerritoryUnited States
CityDenver, CO
Period21/06/0925/06/09

Keywords

  • DNA manipulation
  • DNA stretching
  • Optically-induced dielectrophoresis

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