A novel approach to practical enzymology teaching: A conductimetric investigation of arginase, inorganic pyrophosphatase, aliphatic esterase, ornithine carbamyl transferase and argininosuccinate lyase activities from mammalian liver

Lawrence Anthony; Maclean Andrew; John Ding E. Young; Stevenson Robin

doi:10.1016/S0307-4412(97)00129-5

A novel approach to practical enzymology teaching: A conductimetric investigation of arginase, inorganic pyrophosphatase, aliphatic esterase, ornithine carbamyl transferase and argininosuccinate lyase activities from mammalian liver

Lawrence Anthony
, Maclean Andrew
, John Ding E. Young
, Stevenson Robin

Research output: Contribution to journal › Journal Article › peer-review

Abstract

Kinetic conductivity measurement, run as a microcomputer-controlled workstation with eight independent reaction cells, is shown here to be a simple, cost-effective method for assaying enzymes. The technique is demonstrated with enzymes that can be used as markers for the major subcellular compartments of hepatocytes as a one-day student laboratory class with great potential for variation and development. The method is virtually unknown in enzymology, but could be envisaged as standard apparatus for all biochemical laboratories and teaching classes.

Original language	American English
Pages (from-to)	56-62
Journal	Biochemical Education
Volume	26
Issue number	1
DOIs	https://doi.org/10.1016/S0307-4412(97)00129-5
State	Published - 1998

Keywords

computer program
enzyme activity
enzyme assay
enzymology
medical education
medical student
teaching

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10.1016/S0307-4412(97)00129-5

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Anthony, L., Andrew, M., Young, J. D. E., & Robin, S. (1998). A novel approach to practical enzymology teaching: A conductimetric investigation of arginase, inorganic pyrophosphatase, aliphatic esterase, ornithine carbamyl transferase and argininosuccinate lyase activities from mammalian liver. Biochemical Education, 26(1), 56-62. https://doi.org/10.1016/S0307-4412(97)00129-5

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title = "A novel approach to practical enzymology teaching: A conductimetric investigation of arginase, inorganic pyrophosphatase, aliphatic esterase, ornithine carbamyl transferase and argininosuccinate lyase activities from mammalian liver",

abstract = "Kinetic conductivity measurement, run as a microcomputer-controlled workstation with eight independent reaction cells, is shown here to be a simple, cost-effective method for assaying enzymes. The technique is demonstrated with enzymes that can be used as markers for the major subcellular compartments of hepatocytes as a one-day student laboratory class with great potential for variation and development. The method is virtually unknown in enzymology, but could be envisaged as standard apparatus for all biochemical laboratories and teaching classes.",

keywords = "computer program, enzyme activity, enzyme assay, enzymology, medical education, medical student, teaching",

author = "Lawrence Anthony and Maclean Andrew and Young, \{John Ding E.\} and Stevenson Robin",

year = "1998",

doi = "10.1016/S0307-4412(97)00129-5",

language = "American English",

volume = "26",

pages = "56--62",

journal = "Biochemical Education",

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publisher = "American Society for Biochemistry and Molecular Biology Inc.",

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Anthony, L, Andrew, M, Young, JDE & Robin, S 1998, 'A novel approach to practical enzymology teaching: A conductimetric investigation of arginase, inorganic pyrophosphatase, aliphatic esterase, ornithine carbamyl transferase and argininosuccinate lyase activities from mammalian liver', Biochemical Education, vol. 26, no. 1, pp. 56-62. https://doi.org/10.1016/S0307-4412(97)00129-5

A novel approach to practical enzymology teaching: A conductimetric investigation of arginase, inorganic pyrophosphatase, aliphatic esterase, ornithine carbamyl transferase and argininosuccinate lyase activities from mammalian liver. / Anthony, Lawrence; Andrew, Maclean; Young, John Ding E. et al.
In: Biochemical Education, Vol. 26, No. 1, 1998, p. 56-62.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - A novel approach to practical enzymology teaching: A conductimetric investigation of arginase, inorganic pyrophosphatase, aliphatic esterase, ornithine carbamyl transferase and argininosuccinate lyase activities from mammalian liver

AU - Anthony, Lawrence

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AU - Young, John Ding E.

AU - Robin, Stevenson

PY - 1998

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N2 - Kinetic conductivity measurement, run as a microcomputer-controlled workstation with eight independent reaction cells, is shown here to be a simple, cost-effective method for assaying enzymes. The technique is demonstrated with enzymes that can be used as markers for the major subcellular compartments of hepatocytes as a one-day student laboratory class with great potential for variation and development. The method is virtually unknown in enzymology, but could be envisaged as standard apparatus for all biochemical laboratories and teaching classes.

AB - Kinetic conductivity measurement, run as a microcomputer-controlled workstation with eight independent reaction cells, is shown here to be a simple, cost-effective method for assaying enzymes. The technique is demonstrated with enzymes that can be used as markers for the major subcellular compartments of hepatocytes as a one-day student laboratory class with great potential for variation and development. The method is virtually unknown in enzymology, but could be envisaged as standard apparatus for all biochemical laboratories and teaching classes.

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KW - enzyme activity

KW - enzyme assay

KW - enzymology

KW - medical education

KW - medical student

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DO - 10.1016/S0307-4412(97)00129-5

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