A novel eco-friendly electrochemical fabrication of magnetic bimetallic-organic framework sensors for ultrasensitive detection of dual miRNAs

Muhammad Faizan, Chi Hsien Liu*, Wei Chi Wu

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

Abstract

We developed an eco-friendly method for synthesizing magnetic bimetallic-organic frameworks (MBOF) and applied it to an electrochemical sensor. The MBOF was synthesized using a simple electrochemical approach, helping to reduce the environmental impact of the manufacture. This electrosynthesis utilizes non-toxic salts, recyclable zinc electrodes, and mild reactions. By optimizing parameters such as current density, cathode material, electrolyte, and secondary metal ions, the yield of the MBOF was enhanced. Additionally, the crystal characteristics, chemical structure, and surface morphology of the MBOF can be adjusted by alternating the order of adding organic linkers and metal precursors. The MBOF was characterized through transmission electron microscopy, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction analysis. Anodic stripping voltammetry and ICP-AES were used to detect the incorporation of zinc and copper ions into the MBOF and to comprehensively evaluate the physical and chemical properties of this nanomaterial. The MBOF can be facilly modified with capture probes to detect two types of miRNA. This MBOF-based sensor exhibited a low detection limit of 0.018 fM for miRNA-21 and 0.068 fM for miRNA-3960, with a linear range extending from 0.01 fM to over 100 fM. This system demonstrated rapid analysis of multiple targets in serum, with excellent selectivity and stability, making it highly suitable for point-of-care diagnostics. This platform can also be applied for monitoring RNA or DNA viruses in the environment because of its sensitive detection of trace nucleotide sequences.

Original languageEnglish
Article number115265
JournalJournal of Environmental Chemical Engineering
Volume13
Issue number1
DOIs
StatePublished - 02 2025

Bibliographical note

Publisher Copyright:
© 2025 Elsevier Ltd

Keywords

  • Electrosynthesis
  • Magnetic bimetallic-organic frameworks
  • MiRNA
  • Sensor

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