A novel p53 paralogue mediates antioxidant defense of mosquito cells to survive dengue virus replication

Tien Huang Chen, Yi Jun Wu, Jiun Nan Hou, Yi Hsuan Chiang, Chih Chieh Cheng, Eny Sifiyatun, Cheng Hsun Chiu, Lian Chen Wang*, Wei June Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

5 Scopus citations

Abstract

Mosquito cells allow dengue viruses (DENVs) to undergo replication without causing serious deleterious effects on the cells, leading to advantages for dissemination to other cells. Despite this, increased accumulation of reactive oxygen species (ROS) is usually detected in C6/36 cells with DENV2 infection as shown in mammalian cells. Uniquely, oxidative stress caused by the ROS is alleviated by eliciting antioxidant defense which leads to protection of mosquito cells from the infection. In the present study, a novel p53 paralogue (p53-2) was identified and proved to be regulated in C6/36 cells with DENV2 infection. With a gene-knockdown technique, p53-2 was demonstrated to transcribe catalase which plays a critical role in reducing ROS accumulation and the death rate of infected cells. Ecologically, a higher survival rate of mosquito cells is a prerequisite for prosperous production of viral progeny, allowing infected mosquitoes to remain healthy and active for virus transmission.

Original languageEnglish
Pages (from-to)156-169
Number of pages14
JournalVirology
Volume519
DOIs
StatePublished - 06 2018

Bibliographical note

Publisher Copyright:
© 2018 Elsevier Inc.

Keywords

  • Antioxidant defense
  • Cell survival
  • Dengue 2 virus
  • Gene regulation
  • Mosquito cells
  • P53 paralogue
  • P53-2

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