A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

Chien Neng Kuo; Lin Cheng Yang; Cheng Ta Yang; Miao Fen Chen; Chien Hsiung Lai; Yi Hao Chen; Ching Hsein Chen; Chi Hung Chen; Pei Chang Wu; Hsi Kung Kou; Jen Chia Tsai; Chia Hui Hung

doi:10.1080/02713680802382963

A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

Chien Neng Kuo, Lin Cheng Yang, Cheng Ta Yang, Miao Fen Chen, Chien Hsiung Lai, Yi Hao Chen, Ching Hsein Chen, Chi Hung Chen, Pei Chang Wu, Hsi Kung Kou, Jen Chia Tsai, Chia Hui Hung^*

^*Corresponding author for this work

School of Traditional Chinese Medicine

Research output: Contribution to journal › Journal Article › peer-review

4 Scopus citations

Abstract

Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μg of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μg, 2 μg, or 20 μg of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μg p-bFGF group, days 12-18 in the 2-μg p-bFGF group, and on days 9-15 in the 0.2-μg p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.

Original language	English
Pages (from-to)	839-848
Number of pages	10
Journal	Current Eye Research
Volume	33
Issue number	10
DOIs	https://doi.org/10.1080/02713680802382963
State	Published - 10 2008

Keywords

Angiogenesis
Cornea
Fibroblast growth factor
Nonviral vector
SAINT

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10.1080/02713680802382963

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Kuo, C. N., Yang, L. C., Yang, C. T., Chen, M. F., Lai, C. H., Chen, Y. H., Chen, C. H., Chen, C. H., Wu, P. C., Kou, H. K., Tsai, J. C., & Hung, C. H. (2008). A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Current Eye Research, 33(10), 839-848. https://doi.org/10.1080/02713680802382963

@article{f3e22a2ab55b4871ac5c645e051884c0,

title = "A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex",

abstract = "Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μg of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μg, 2 μg, or 20 μg of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μg p-bFGF group, days 12-18 in the 2-μg p-bFGF group, and on days 9-15 in the 0.2-μg p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.",

keywords = "Angiogenesis, Cornea, Fibroblast growth factor, Nonviral vector, SAINT",

author = "Kuo, {Chien Neng} and Yang, {Lin Cheng} and Yang, {Cheng Ta} and Chen, {Miao Fen} and Lai, {Chien Hsiung} and Chen, {Yi Hao} and Chen, {Ching Hsein} and Chen, {Chi Hung} and Wu, {Pei Chang} and Kou, {Hsi Kung} and Tsai, {Jen Chia} and Hung, {Chia Hui}",

year = "2008",

month = oct,

doi = "10.1080/02713680802382963",

language = "英语",

volume = "33",

pages = "839--848",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Taylor and Francis Ltd.",

number = "10",

}

Kuo, CN, Yang, LC, Yang, CT, Chen, MF, Lai, CH, Chen, YH, Chen, CH, Chen, CH, Wu, PC, Kou, HK, Tsai, JC & Hung, CH 2008, 'A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex', Current Eye Research, vol. 33, no. 10, pp. 839-848. https://doi.org/10.1080/02713680802382963

A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex. / Kuo, Chien Neng; Yang, Lin Cheng; Yang, Cheng Ta et al.
In: Current Eye Research, Vol. 33, No. 10, 10.2008, p. 839-848.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

AU - Kuo, Chien Neng

AU - Yang, Lin Cheng

AU - Yang, Cheng Ta

AU - Chen, Miao Fen

AU - Lai, Chien Hsiung

AU - Chen, Yi Hao

AU - Chen, Ching Hsein

AU - Chen, Chi Hung

AU - Wu, Pei Chang

AU - Kou, Hsi Kung

AU - Tsai, Jen Chia

AU - Hung, Chia Hui

PY - 2008/10

Y1 - 2008/10

N2 - Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μg of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μg, 2 μg, or 20 μg of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μg p-bFGF group, days 12-18 in the 2-μg p-bFGF group, and on days 9-15 in the 0.2-μg p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.

AB - Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μg of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μg, 2 μg, or 20 μg of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μg p-bFGF group, days 12-18 in the 2-μg p-bFGF group, and on days 9-15 in the 0.2-μg p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.

KW - Angiogenesis

KW - Cornea

KW - Fibroblast growth factor

KW - Nonviral vector

KW - SAINT

UR - http://www.scopus.com/inward/record.url?scp=53849092734&partnerID=8YFLogxK

U2 - 10.1080/02713680802382963

DO - 10.1080/02713680802382963

M3 - 文章

C2 - 18853317

AN - SCOPUS:53849092734

SN - 0271-3683

VL - 33

SP - 839

EP - 848

JO - Current Eye Research

JF - Current Eye Research

IS - 10

ER -

A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

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