A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

Chien Neng Kuo; Lin Cheng Yang; Cheng Ta Yang; Miao Fen Chen; Chien Hsiung Lai; Yi Hao Chen; Ching Hsein Chen; Chi Hung Chen; Pei Chang Wu; Hsi Kung Kou; Jen Chia Tsai; Chia Hui Hung

doi:10.1080/02713680802382963

A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

Chien Neng Kuo
, Lin Cheng Yang
, Cheng Ta Yang
, Miao Fen Chen
, Chien Hsiung Lai
, Yi Hao Chen
, Ching Hsein Chen
, Chi Hung Chen
, Pei Chang Wu
, Hsi Kung Kou
, Jen Chia Tsai
, Chia Hui Hung^*

^*Corresponding author for this work

School of Traditional Chinese Medicine

Chang Gung Memorial Hospital
Chang Gung University
I-Shou University
National Chiayi University
Chia-Yi Christian Hospital

Research output: Contribution to journal › Journal Article › peer-review

4 Scopus citations

Abstract

Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μg of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μg, 2 μg, or 20 μg of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μg p-bFGF group, days 12-18 in the 2-μg p-bFGF group, and on days 9-15 in the 0.2-μg p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.

Original language	English
Pages (from-to)	839-848
Number of pages	10
Journal	Current Eye Research
Volume	33
Issue number	10
DOIs	https://doi.org/10.1080/02713680802382963
State	Published - 10 2008

Keywords

Angiogenesis
Cornea
Fibroblast growth factor
Nonviral vector
SAINT

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10.1080/02713680802382963

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Kuo, C. N., Yang, L. C., Yang, C. T., Chen, M. F., Lai, C. H., Chen, Y. H., Chen, C. H., Chen, C. H., Wu, P. C., Kou, H. K., Tsai, J. C., & Hung, C. H. (2008). A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Current Eye Research, 33(10), 839-848. https://doi.org/10.1080/02713680802382963

@article{f3e22a2ab55b4871ac5c645e051884c0,

title = "A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex",

abstract = "Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μg of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μg, 2 μg, or 20 μg of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μg p-bFGF group, days 12-18 in the 2-μg p-bFGF group, and on days 9-15 in the 0.2-μg p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.",

keywords = "Angiogenesis, Cornea, Fibroblast growth factor, Nonviral vector, SAINT",

author = "Kuo, \{Chien Neng\} and Yang, \{Lin Cheng\} and Yang, \{Cheng Ta\} and Chen, \{Miao Fen\} and Lai, \{Chien Hsiung\} and Chen, \{Yi Hao\} and Chen, \{Ching Hsein\} and Chen, \{Chi Hung\} and Wu, \{Pei Chang\} and Kou, \{Hsi Kung\} and Tsai, \{Jen Chia\} and Hung, \{Chia Hui\}",

year = "2008",

month = oct,

doi = "10.1080/02713680802382963",

language = "英语",

volume = "33",

pages = "839--848",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Taylor and Francis Ltd.",

number = "10",

}

Kuo, CN, Yang, LC, Yang, CT, Chen, MF, Lai, CH, Chen, YH, Chen, CH, Chen, CH, Wu, PC, Kou, HK, Tsai, JC & Hung, CH 2008, 'A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex', Current Eye Research, vol. 33, no. 10, pp. 839-848. https://doi.org/10.1080/02713680802382963

A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex. / Kuo, Chien Neng; Yang, Lin Cheng; Yang, Cheng Ta et al.
In: Current Eye Research, Vol. 33, No. 10, 10.2008, p. 839-848.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

AU - Kuo, Chien Neng

AU - Yang, Lin Cheng

AU - Yang, Cheng Ta

AU - Chen, Miao Fen

AU - Lai, Chien Hsiung

AU - Chen, Yi Hao

AU - Chen, Ching Hsein

AU - Chen, Chi Hung

AU - Wu, Pei Chang

AU - Kou, Hsi Kung

AU - Tsai, Jen Chia

AU - Hung, Chia Hui

PY - 2008/10

Y1 - 2008/10

N2 - Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μg of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μg, 2 μg, or 20 μg of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μg p-bFGF group, days 12-18 in the 2-μg p-bFGF group, and on days 9-15 in the 0.2-μg p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.

AB - Purpose: We describe a novel vector system of nonviral gene transfer into the cornea using a partially dried form of a plasmid expressing 18-kDa basic fibroblast growth factor (p-bFGF)-synthetic amphiphile INTeraction-18 (SAINT-18) complex. Methods: Corneal neovascularization (NV) was evaluated in 48 eyes of Sprague-Dawley rats after implantation of SAINT-18 containing 2 μg of plasmid-expressing green fluorescent protein (p-GFP; control group), 0.2 μg, 2 μg, or 20 μg of p-bFGF from day 0 to day 60. bFGF protein expression was analyzed by Western blotting and immunohistochemistry. Results: The p-bFGF-SAINT-18 complex induced dose-dependent corneal neovascularization, which reached a maximum on days 15-21 in the 20-μg p-bFGF group, days 12-18 in the 2-μg p-bFGF group, and on days 9-15 in the 0.2-μg p-bFGF group, and then regressed progressively. No NV was observed in the p-GFP group. Conclusions: This noninflammatory corneal transfection model using partially dried p-bFGF-SAINT-18 complex allows precise localization of tranfection reagents for producing corneal neovascularization.

KW - Angiogenesis

KW - Cornea

KW - Fibroblast growth factor

KW - Nonviral vector

KW - SAINT

UR - https://www.scopus.com/pages/publications/53849092734

U2 - 10.1080/02713680802382963

DO - 10.1080/02713680802382963

M3 - 文章

C2 - 18853317

AN - SCOPUS:53849092734

SN - 0271-3683

VL - 33

SP - 839

EP - 848

JO - Current Eye Research

JF - Current Eye Research

IS - 10

ER -

A novel vector system for gene transfer into the cornea using a partially dried plasmid expressing 18 basic fibroblast growth factor-synthetic amphiphile INTeraction-18 (SAINT-18) complex

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Keywords

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