A perfusion 3D cell culture biochip with on-chip vertical electrodes for detecting cell number by electrical impedance measurement

Che Wei Hsu, Kin Fong Lei*, Cheng Yuan Lin, Min Hsien Wu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A perfusion three dimensional (3D) cell culture biochip embedded with on-chip vertical electrodes has been developed and is capable of onsite detecting the cell number in the 3D cell culture construct without sacrificing the cultured cells. Conventional monitoring of the cell number in 3D cell culture construct is normally based on the detection of the indicative cellular components (e.g., DNA assay), which requires sacrificing the cultured cells. In this study, the proposed biochip provides a well-defined and homogenous perfusion 3D culture environment and onsite monitoring of the cell number in the 3D culture construct by impedance measurement.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages479-481
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 10 201201 11 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/1201/11/12

Keywords

  • Biochip
  • Cell culture
  • Cell number
  • Impedance measurement
  • Vertical electrode

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