TY - JOUR
T1 - A phase I study of autologous dendritic cell-based immunotherapy for patients with unresectable primary liver cancer
AU - Iwashita, Yukio
AU - Tahara, Kouichirou
AU - Goto, Shigeru
AU - Sasaki, Atsushi
AU - Kai, Seiichiro
AU - Seike, Masataka
AU - Chen, Chao Long
AU - Kawano, Katsunori
AU - Kitano, Seigo
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Background/aims: To evaluate the safety and feasibility of immunotherapy based on autologous dendritic cells (DC) for patients with unresectable primary liver cancer (PLC). Methods: A total of ten patients were enrolled and immunized with DCs. Autologous DCs were generated ex vivo in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Cells were then pulsed with tumor lysate (TL), tumor necrosis factor-α (TNF-α) and keyhole limpet hemocyanin (KLH). Non-adherent cells were collected on day 9 and cells were administered into the inguinal lymph node. Each patient received 1-10 × 106 cells four times at weekly intervals. Results: Immunization was well tolerated in all patients without significant toxicity. DC vaccination induced delayed-type hypersensitivity (DTH) against KLH in seven out of ten patients. In one patient, one of the two liver tumors (tumor in segment 7, 13 mm in diameter) decreased in size to 7 mm and showed necrotic change on computed tomography examination after eight immunizations. In two patients, serum levels of tumor markers decreased after vaccination. Conclusion: The present clinical trial suggested that immunization by TL-pulsed DCs is feasible in patients with unresectable PLC without any toxicity. Further improvement in the clinical results of immunotherapy might be expected by modifying the therapeutic protocol.
AB - Background/aims: To evaluate the safety and feasibility of immunotherapy based on autologous dendritic cells (DC) for patients with unresectable primary liver cancer (PLC). Methods: A total of ten patients were enrolled and immunized with DCs. Autologous DCs were generated ex vivo in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Cells were then pulsed with tumor lysate (TL), tumor necrosis factor-α (TNF-α) and keyhole limpet hemocyanin (KLH). Non-adherent cells were collected on day 9 and cells were administered into the inguinal lymph node. Each patient received 1-10 × 106 cells four times at weekly intervals. Results: Immunization was well tolerated in all patients without significant toxicity. DC vaccination induced delayed-type hypersensitivity (DTH) against KLH in seven out of ten patients. In one patient, one of the two liver tumors (tumor in segment 7, 13 mm in diameter) decreased in size to 7 mm and showed necrotic change on computed tomography examination after eight immunizations. In two patients, serum levels of tumor markers decreased after vaccination. Conclusion: The present clinical trial suggested that immunization by TL-pulsed DCs is feasible in patients with unresectable PLC without any toxicity. Further improvement in the clinical results of immunotherapy might be expected by modifying the therapeutic protocol.
KW - Cell therapy
KW - Cholangiocellular carcinoma
KW - Delayed-type hypersensitivity
KW - Hepatocellular carcinoma
KW - Keyhole limpet hemocyanin
UR - http://www.scopus.com/inward/record.url?scp=0037384518&partnerID=8YFLogxK
U2 - 10.1007/s00262-002-0360-9
DO - 10.1007/s00262-002-0360-9
M3 - 文章
C2 - 12649744
AN - SCOPUS:0037384518
SN - 0340-7004
VL - 52
SP - 155
EP - 161
JO - Cancer Immunology Immunotherapy
JF - Cancer Immunology Immunotherapy
IS - 3
ER -
