A potential acyltransferase regulates swarming in Serratia marcescens

Po Chi Soo; Yu Tze Horng; Ying Hsien Fu; Chia Chen Lu; Hsin Chih Lai

doi:10.1016/j.bbrc.2008.04.109

A potential acyltransferase regulates swarming in Serratia marcescens

Po Chi Soo, Yu Tze Horng, Ying Hsien Fu, Chia Chen Lu, Hsin Chih Lai^*

^*Corresponding author for this work

Department of Medical Biotechnology and Laboratory Science

Research output: Contribution to journal › Journal Article › peer-review

5 Scopus citations

Abstract

Swarming in Serratia marcescens is a specialized form of bacterial populational surface migration behavior. A precocious swarming mutant S. marcescens O6 whose rssC is interrupted by mini-Tn5 transposon was obtained. The 364 amino-acid RssC showed a high identity to members of acyltransferase family. Characterization of the rssC knock-out mutant S. marcescens PC105 showed aberrant and hypervirulent phenotypes including precocious swarming behavior, reduced cell attachment ability and increase in swimming motility, flagellin synthesis, capsular polysaccharide (CPS) production, hemolysin activity, and cytotoxicity activity. Besides a more intense pattern of O-antigen sugar unit ladder, S. marcescens PC105 also showed an altered cell surface topology. A potential FlhDC binding site was identified at the rssC promoter region. In accordance, rssC transcriptional activity was up-regulated by FlhDC_Sm. Briefly, rssC is hierarchically classified as class II members in FlhDC_Sm regulon, and reciprocally defect in rssC results in aberrant swarming and hypervirulence in S. marcescens.

Original language	English
Pages (from-to)	462-467
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	371
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2008.04.109
State	Published - 04 07 2008

Keywords

Acyltransferase
Cytotoxicity
Flagellin
FlhDC
Hemolysin
Polysaccharide
Serratia
Swarming

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10.1016/j.bbrc.2008.04.109

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title = "A potential acyltransferase regulates swarming in Serratia marcescens",

abstract = "Swarming in Serratia marcescens is a specialized form of bacterial populational surface migration behavior. A precocious swarming mutant S. marcescens O6 whose rssC is interrupted by mini-Tn5 transposon was obtained. The 364 amino-acid RssC showed a high identity to members of acyltransferase family. Characterization of the rssC knock-out mutant S. marcescens PC105 showed aberrant and hypervirulent phenotypes including precocious swarming behavior, reduced cell attachment ability and increase in swimming motility, flagellin synthesis, capsular polysaccharide (CPS) production, hemolysin activity, and cytotoxicity activity. Besides a more intense pattern of O-antigen sugar unit ladder, S. marcescens PC105 also showed an altered cell surface topology. A potential FlhDC binding site was identified at the rssC promoter region. In accordance, rssC transcriptional activity was up-regulated by FlhDCSm. Briefly, rssC is hierarchically classified as class II members in FlhDCSm regulon, and reciprocally defect in rssC results in aberrant swarming and hypervirulence in S. marcescens.",

keywords = "Acyltransferase, Cytotoxicity, Flagellin, FlhDC, Hemolysin, Polysaccharide, Serratia, Swarming",

author = "Soo, {Po Chi} and Horng, {Yu Tze} and Fu, {Ying Hsien} and Lu, {Chia Chen} and Lai, {Hsin Chih}",

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AU - Soo, Po Chi

AU - Horng, Yu Tze

AU - Fu, Ying Hsien

AU - Lu, Chia Chen

AU - Lai, Hsin Chih

PY - 2008/7/4

Y1 - 2008/7/4

N2 - Swarming in Serratia marcescens is a specialized form of bacterial populational surface migration behavior. A precocious swarming mutant S. marcescens O6 whose rssC is interrupted by mini-Tn5 transposon was obtained. The 364 amino-acid RssC showed a high identity to members of acyltransferase family. Characterization of the rssC knock-out mutant S. marcescens PC105 showed aberrant and hypervirulent phenotypes including precocious swarming behavior, reduced cell attachment ability and increase in swimming motility, flagellin synthesis, capsular polysaccharide (CPS) production, hemolysin activity, and cytotoxicity activity. Besides a more intense pattern of O-antigen sugar unit ladder, S. marcescens PC105 also showed an altered cell surface topology. A potential FlhDC binding site was identified at the rssC promoter region. In accordance, rssC transcriptional activity was up-regulated by FlhDCSm. Briefly, rssC is hierarchically classified as class II members in FlhDCSm regulon, and reciprocally defect in rssC results in aberrant swarming and hypervirulence in S. marcescens.

AB - Swarming in Serratia marcescens is a specialized form of bacterial populational surface migration behavior. A precocious swarming mutant S. marcescens O6 whose rssC is interrupted by mini-Tn5 transposon was obtained. The 364 amino-acid RssC showed a high identity to members of acyltransferase family. Characterization of the rssC knock-out mutant S. marcescens PC105 showed aberrant and hypervirulent phenotypes including precocious swarming behavior, reduced cell attachment ability and increase in swimming motility, flagellin synthesis, capsular polysaccharide (CPS) production, hemolysin activity, and cytotoxicity activity. Besides a more intense pattern of O-antigen sugar unit ladder, S. marcescens PC105 also showed an altered cell surface topology. A potential FlhDC binding site was identified at the rssC promoter region. In accordance, rssC transcriptional activity was up-regulated by FlhDCSm. Briefly, rssC is hierarchically classified as class II members in FlhDCSm regulon, and reciprocally defect in rssC results in aberrant swarming and hypervirulence in S. marcescens.

KW - Acyltransferase

KW - Cytotoxicity

KW - Flagellin

KW - FlhDC

KW - Hemolysin

KW - Polysaccharide

KW - Serratia

KW - Swarming

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JO - Biochemical and Biophysical Research Communications

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A potential acyltransferase regulates swarming in Serratia marcescens

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