A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP)

Yu Dong Ma; Wen Hsin Chang; Chih Hung Wang; Shih Yuan Liu; Wen Hsiang Yen; Kang Luo; Huey Ling You; Jiunn Jong Wu; Mel S. Lee; Gwo Bin Lee

doi:10.1109/NEMS.2016.7758187

A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP)

Yu Dong Ma, Wen Hsin Chang, Chih Hung Wang, Shih Yuan Liu, Wen Hsiang Yen, Kang Luo, Huey Ling You, Jiunn Jong Wu, Mel S. Lee, Gwo Bin Lee^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

Digital loop-mediated amplification (LAMP) is an isothermal, quantitative DNA amplification approach which has high sensitivity and specificity and could be used to analyze extremely low amount of DNA within a short period of time. In this study, an integrated self-driven microfluidic chip was proposed herein for performing a digital LAMP process for vancomycin-resistant Enterococcus (VRE) diagnosis. The entire bacterial diagnosis could be automatically performed by capillary forces through the new polydimethylsiloxane (PDMS) surface treatment without using external pumps. Moreover, digitized droplets could be separated from each other by normally-close valves such that it could be quantitated. The contact angle of the treated PDMS surface was measured to be only 24°, which was the lowest value in literatures. This is the first time that a rapid (within 1 hour), simple, and hydrophilic PDMS surface modification method has been reported, which could be used in self-driven microfluidic devices for digital LAMP. This device may become a promising tool for clinical diagnosis and point-of-care applications.

Original language	English
Title of host publication	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1-4
Number of pages	4
ISBN (Electronic)	9781509019472
DOIs	https://doi.org/10.1109/NEMS.2016.7758187
State	Published - 28 11 2016
Externally published	Yes
Event	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan Duration: 17 04 2016 → 20 04 2016

Publication series

Name	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Conference

Conference	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Country/Territory	Japan
City	Sendai
Period	17/04/16 → 20/04/16

Bibliographical note

Publisher Copyright:
© 2016 IEEE.

Keywords

PDMS modification
VRE
digital LAMP
microfluidics

Access to Document

10.1109/NEMS.2016.7758187

Cite this

Ma, Y. D., Chang, W. H., Wang, C. H., Liu, S. Y., Yen, W. H., Luo, K., You, H. L., Wu, J. J., Lee, M. S., & Lee, G. B. (2016). A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP). In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 (pp. 1-4). Article 7758187 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2016.7758187

Ma, Yu Dong ; Chang, Wen Hsin ; Wang, Chih Hung et al. / A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP). 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 1-4 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

@inproceedings{895a7214a6634b4fb4f8218968ada207,

title = "A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP)",

abstract = "Digital loop-mediated amplification (LAMP) is an isothermal, quantitative DNA amplification approach which has high sensitivity and specificity and could be used to analyze extremely low amount of DNA within a short period of time. In this study, an integrated self-driven microfluidic chip was proposed herein for performing a digital LAMP process for vancomycin-resistant Enterococcus (VRE) diagnosis. The entire bacterial diagnosis could be automatically performed by capillary forces through the new polydimethylsiloxane (PDMS) surface treatment without using external pumps. Moreover, digitized droplets could be separated from each other by normally-close valves such that it could be quantitated. The contact angle of the treated PDMS surface was measured to be only 24°, which was the lowest value in literatures. This is the first time that a rapid (within 1 hour), simple, and hydrophilic PDMS surface modification method has been reported, which could be used in self-driven microfluidic devices for digital LAMP. This device may become a promising tool for clinical diagnosis and point-of-care applications.",

keywords = "PDMS modification, VRE, digital LAMP, microfluidics",

author = "Ma, {Yu Dong} and Chang, {Wen Hsin} and Wang, {Chih Hung} and Liu, {Shih Yuan} and Yen, {Wen Hsiang} and Kang Luo and You, {Huey Ling} and Wu, {Jiunn Jong} and Lee, {Mel S.} and Lee, {Gwo Bin}",

note = "Publisher Copyright: {\textcopyright} 2016 IEEE.; 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 ; Conference date: 17-04-2016 Through 20-04-2016",

year = "2016",

month = nov,

day = "28",

doi = "10.1109/NEMS.2016.7758187",

language = "英语",

series = "2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "1--4",

booktitle = "2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016",

address = "美国",

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Ma, YD, Chang, WH, Wang, CH, Liu, SY, Yen, WH, Luo, K, You, HL, Wu, JJ, Lee, MS & Lee, GB 2016, A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP). in 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016., 7758187, 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Institute of Electrical and Electronics Engineers Inc., pp. 1-4, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Sendai, Japan, 17/04/16. https://doi.org/10.1109/NEMS.2016.7758187

A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP). / Ma, Yu Dong; Chang, Wen Hsin; Wang, Chih Hung et al.
2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 1-4 7758187 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP)

AU - Ma, Yu Dong

AU - Chang, Wen Hsin

AU - Wang, Chih Hung

AU - Liu, Shih Yuan

AU - Yen, Wen Hsiang

AU - Luo, Kang

AU - You, Huey Ling

AU - Wu, Jiunn Jong

AU - Lee, Mel S.

AU - Lee, Gwo Bin

PY - 2016/11/28

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N2 - Digital loop-mediated amplification (LAMP) is an isothermal, quantitative DNA amplification approach which has high sensitivity and specificity and could be used to analyze extremely low amount of DNA within a short period of time. In this study, an integrated self-driven microfluidic chip was proposed herein for performing a digital LAMP process for vancomycin-resistant Enterococcus (VRE) diagnosis. The entire bacterial diagnosis could be automatically performed by capillary forces through the new polydimethylsiloxane (PDMS) surface treatment without using external pumps. Moreover, digitized droplets could be separated from each other by normally-close valves such that it could be quantitated. The contact angle of the treated PDMS surface was measured to be only 24°, which was the lowest value in literatures. This is the first time that a rapid (within 1 hour), simple, and hydrophilic PDMS surface modification method has been reported, which could be used in self-driven microfluidic devices for digital LAMP. This device may become a promising tool for clinical diagnosis and point-of-care applications.

AB - Digital loop-mediated amplification (LAMP) is an isothermal, quantitative DNA amplification approach which has high sensitivity and specificity and could be used to analyze extremely low amount of DNA within a short period of time. In this study, an integrated self-driven microfluidic chip was proposed herein for performing a digital LAMP process for vancomycin-resistant Enterococcus (VRE) diagnosis. The entire bacterial diagnosis could be automatically performed by capillary forces through the new polydimethylsiloxane (PDMS) surface treatment without using external pumps. Moreover, digitized droplets could be separated from each other by normally-close valves such that it could be quantitated. The contact angle of the treated PDMS surface was measured to be only 24°, which was the lowest value in literatures. This is the first time that a rapid (within 1 hour), simple, and hydrophilic PDMS surface modification method has been reported, which could be used in self-driven microfluidic devices for digital LAMP. This device may become a promising tool for clinical diagnosis and point-of-care applications.

KW - PDMS modification

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M3 - 会议稿件

AN - SCOPUS:85007198680

T3 - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

SP - 1

EP - 4

BT - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

PB - Institute of Electrical and Electronics Engineers Inc.

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ER -

Ma YD, Chang WH, Wang CH, Liu SY, Yen WH, Luo K et al. A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP). In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 1-4. 7758187. (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). doi: 10.1109/NEMS.2016.7758187

A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP)

Abstract

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Keywords

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