A simple method to determine urea concentration using intact Helicobacter pylori and BromoCresol Purple as a pH indicator

Yuh Ling Lin; Chien Tsu Chen; Su Cheng Lin; Cheng Lee; Hsien Shou Kuo; Chun Mean Shih; Yuan Hsun Hsu; Yi Ping Chin; Err Cheng Chan

doi:10.1023/A:1005614501420

A simple method to determine urea concentration using intact Helicobacter pylori and BromoCresol Purple as a pH indicator

Yuh Ling Lin^*, Chien Tsu Chen, Su Cheng Lin, Cheng Lee, Hsien Shou Kuo, Chun Mean Shih, Yuan Hsun Hsu, Yi Ping Chin, Err Cheng Chan

^*Corresponding author for this work

Department of Medical Biotechnology and Laboratory Science

Taipei Medical University

Research output: Contribution to journal › Journal Article › peer-review

5 Scopus citations

Abstract

A modified method for urea quantification, by measuring the ammonia formed by urease, used the urease-positive Helicobacter pylori in place of purified urease with a pH indicator dye, BromoCresol Purple, to provide a color change. The color formed was stable for 20 min and could be read at 588 nm for urea quantification. Using this method, urea standard curves were linear up to 8.3 mM. As there was no need for centrifugation or precipitation, the assay was developed for use with 96-well microplates.

Original language	English
Pages (from-to)	1077-1079
Number of pages	3
Journal	Biotechnology Letters
Volume	22
Issue number	13
DOIs	https://doi.org/10.1023/A:1005614501420
State	Published - 2000

Keywords

Ammonia
BromoCresol Purple
Helicobacter pylori
Urea
Urease

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title = "A simple method to determine urea concentration using intact Helicobacter pylori and BromoCresol Purple as a pH indicator",

abstract = "A modified method for urea quantification, by measuring the ammonia formed by urease, used the urease-positive Helicobacter pylori in place of purified urease with a pH indicator dye, BromoCresol Purple, to provide a color change. The color formed was stable for 20 min and could be read at 588 nm for urea quantification. Using this method, urea standard curves were linear up to 8.3 mM. As there was no need for centrifugation or precipitation, the assay was developed for use with 96-well microplates.",

keywords = "Ammonia, BromoCresol Purple, Helicobacter pylori, Urea, Urease",

author = "Lin, {Yuh Ling} and Chen, {Chien Tsu} and Lin, {Su Cheng} and Cheng Lee and Kuo, {Hsien Shou} and Shih, {Chun Mean} and Hsu, {Yuan Hsun} and Chin, {Yi Ping} and Chan, {Err Cheng}",

year = "2000",

doi = "10.1023/A:1005614501420",

language = "英语",

volume = "22",

pages = "1077--1079",

journal = "Biotechnology Letters",

issn = "0141-5492",

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TY - JOUR

T1 - A simple method to determine urea concentration using intact Helicobacter pylori and BromoCresol Purple as a pH indicator

AU - Lin, Yuh Ling

AU - Chen, Chien Tsu

AU - Lin, Su Cheng

AU - Lee, Cheng

AU - Kuo, Hsien Shou

AU - Shih, Chun Mean

AU - Hsu, Yuan Hsun

AU - Chin, Yi Ping

AU - Chan, Err Cheng

PY - 2000

Y1 - 2000

N2 - A modified method for urea quantification, by measuring the ammonia formed by urease, used the urease-positive Helicobacter pylori in place of purified urease with a pH indicator dye, BromoCresol Purple, to provide a color change. The color formed was stable for 20 min and could be read at 588 nm for urea quantification. Using this method, urea standard curves were linear up to 8.3 mM. As there was no need for centrifugation or precipitation, the assay was developed for use with 96-well microplates.

AB - A modified method for urea quantification, by measuring the ammonia formed by urease, used the urease-positive Helicobacter pylori in place of purified urease with a pH indicator dye, BromoCresol Purple, to provide a color change. The color formed was stable for 20 min and could be read at 588 nm for urea quantification. Using this method, urea standard curves were linear up to 8.3 mM. As there was no need for centrifugation or precipitation, the assay was developed for use with 96-well microplates.

KW - Ammonia

KW - BromoCresol Purple

KW - Helicobacter pylori

KW - Urea

KW - Urease

UR - http://www.scopus.com/inward/record.url?scp=0033929680&partnerID=8YFLogxK

U2 - 10.1023/A:1005614501420

DO - 10.1023/A:1005614501420

M3 - 文章

AN - SCOPUS:0033929680

SN - 0141-5492

VL - 22

SP - 1077

EP - 1079

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 13

ER -

A simple method to determine urea concentration using intact Helicobacter pylori and BromoCresol Purple as a pH indicator

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Keywords

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