TY - JOUR
T1 - A single-dose of cobalt-protoporphyrin protects islet beta cells from glucocorticoid suppression
AU - Hsu, B. R.S.
AU - Chen, S. T.
AU - Fu, S. H.
PY - 2005/5
Y1 - 2005/5
N2 - This study examined whether treating donor mice with a single-dose of cobalt protoporphyrin (CoPP) could induce heme oxygenase-1 (HO-1) and thus protect islet cells from suppression by high-dose glucocorticoid. Islets were isolated from mice receiving either a single dose of CoPP (20 mg/kg body weight) (CoPP-islets) or isotonic sodium chloride solution (control islets) at 24 hours before isolation. Following incubation in the absence or presence of methylprednisolone (100 and 1000 ng/mL) for 24 hours, glucose-stimulated insulin secretion and insulin content of cultured islets were determined. Data were expressed as the mean ± standard error. HO-1 protein level of CoPP-islets was significantly higher than that of normal islets at 12 hours (P <. 005) and 30 hours (P <. 05) but not at 56 hours after CoPP administration (P = NS). The expression of CPP-32, an apoptosis inducer, was significantly inhibited in CoPP-islets at 24 hours after CoPP administration. Compared to the control islets, CoPP-islets secreted significantly more insulin in response to glucose stimulation following 24-hour incubation with 100 and 1000 ng/mL of methylprednisolone (P <. 05 and P <. 05). The insulin content of both control and CoPP-islets did not differ significantly after 24-hour incubation with methylprednisolone. In conclusion, a single-dose treatment with cobalt-protoporphyrin for the induction of heme oxygenase-1 protects islets against the suppressive effect of methylprednisolone.
AB - This study examined whether treating donor mice with a single-dose of cobalt protoporphyrin (CoPP) could induce heme oxygenase-1 (HO-1) and thus protect islet cells from suppression by high-dose glucocorticoid. Islets were isolated from mice receiving either a single dose of CoPP (20 mg/kg body weight) (CoPP-islets) or isotonic sodium chloride solution (control islets) at 24 hours before isolation. Following incubation in the absence or presence of methylprednisolone (100 and 1000 ng/mL) for 24 hours, glucose-stimulated insulin secretion and insulin content of cultured islets were determined. Data were expressed as the mean ± standard error. HO-1 protein level of CoPP-islets was significantly higher than that of normal islets at 12 hours (P <. 005) and 30 hours (P <. 05) but not at 56 hours after CoPP administration (P = NS). The expression of CPP-32, an apoptosis inducer, was significantly inhibited in CoPP-islets at 24 hours after CoPP administration. Compared to the control islets, CoPP-islets secreted significantly more insulin in response to glucose stimulation following 24-hour incubation with 100 and 1000 ng/mL of methylprednisolone (P <. 05 and P <. 05). The insulin content of both control and CoPP-islets did not differ significantly after 24-hour incubation with methylprednisolone. In conclusion, a single-dose treatment with cobalt-protoporphyrin for the induction of heme oxygenase-1 protects islets against the suppressive effect of methylprednisolone.
UR - http://www.scopus.com/inward/record.url?scp=20344394198&partnerID=8YFLogxK
U2 - 10.1016/j.transproceed.2005.02.085
DO - 10.1016/j.transproceed.2005.02.085
M3 - 文章
C2 - 15919478
AN - SCOPUS:20344394198
SN - 0041-1345
VL - 37
SP - 1826
EP - 1827
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 4
ER -