A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer

Ying Ju Chen, Yen Heng Lin*, Chao Sung Lai, Yi Ting Chen, Jau Song Yu, Yu Sun Chang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

This paper reports a negative-pressure driven microfluidic chip, which is capable of rapidly detecting bladder cancer bi-omarker, APOA1, with aid of magnetic beads-based Enzyme-linked immunosorbent assays (ELISA). Fluid can be handled through vacuum force and normally-closed valve. Comparing the experimental results between integrated chip and 96 well plate, the former requires less time (4 hr to 30 min), fewer reagent consumption (100 μl to 30 μl). In this study, the beads-based ELISA in chip has higher detection range and shows better performance in both sensitivity and linearity(99.2%) than conventional ELISA in plate.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1692-1694
Number of pages3
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 02 10 201106 10 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period02/10/1106/10/11

Keywords

  • Apolipoprotein A-I (APOA1)
  • Bladder cancer
  • ELISA
  • Magnetic beads
  • Microfluidic chip

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