A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer

Ying Ju Chen; Yen Heng Lin; Chao Sung Lai; Yi Ting Chen; Jau Song Yu; Yu Sun Chang

A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer

Ying Ju Chen
, Yen Heng Lin^*
, Chao Sung Lai
, Yi Ting Chen
, Jau Song Yu
, Yu Sun Chang

^*Corresponding author for this work

Chang Gung University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

This paper reports a negative-pressure driven microfluidic chip, which is capable of rapidly detecting bladder cancer bi-omarker, APOA1, with aid of magnetic beads-based Enzyme-linked immunosorbent assays (ELISA). Fluid can be handled through vacuum force and normally-closed valve. Comparing the experimental results between integrated chip and 96 well plate, the former requires less time (4 hr to 30 min), fewer reagent consumption (100 μl to 30 μl). In this study, the beads-based ELISA in chip has higher detection range and shows better performance in both sensitivity and linearity(99.2%) than conventional ELISA in plate.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	1692-1694
Number of pages	3
State	Published - 2011
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 02 10 2011 → 06 10 2011

Publication series

Name	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume	3

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/Territory	United States
City	Seattle, WA
Period	02/10/11 → 06/10/11

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apolipoprotein A-I (APOA1)
Bladder cancer
ELISA
Magnetic beads
Microfluidic chip

Cite this

Chen, Y. J., Lin, Y. H., Lai, C. S., Chen, Y. T., Yu, J. S., & Chang, Y. S. (2011). A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1692-1694). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Chen, Ying Ju ; Lin, Yen Heng ; Lai, Chao Sung et al. / A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1692-1694 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).

@inproceedings{9b1ad3b76c80432390d59f0257ff57cc,

title = "A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer",

abstract = "This paper reports a negative-pressure driven microfluidic chip, which is capable of rapidly detecting bladder cancer bi-omarker, APOA1, with aid of magnetic beads-based Enzyme-linked immunosorbent assays (ELISA). Fluid can be handled through vacuum force and normally-closed valve. Comparing the experimental results between integrated chip and 96 well plate, the former requires less time (4 hr to 30 min), fewer reagent consumption (100 μl to 30 μl). In this study, the beads-based ELISA in chip has higher detection range and shows better performance in both sensitivity and linearity(99.2\%) than conventional ELISA in plate.",

keywords = "Apolipoprotein A-I (APOA1), Bladder cancer, ELISA, Magnetic beads, Microfluidic chip",

author = "Chen, \{Ying Ju\} and Lin, \{Yen Heng\} and Lai, \{Chao Sung\} and Chen, \{Yi Ting\} and Yu, \{Jau Song\} and Chang, \{Yu Sun\}",

year = "2011",

language = "英语",

isbn = "9781618395955",

series = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

pages = "1692--1694",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

}

Chen, YJ, Lin, YH, Lai, CS, Chen, YT , Yu, JS & Chang, YS 2011, A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 3, pp. 1692-1694, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 02/10/11.

A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer. / Chen, Ying Ju; Lin, Yen Heng; Lai, Chao Sung et al.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1692-1694 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer

AU - Chen, Ying Ju

AU - Lin, Yen Heng

AU - Lai, Chao Sung

AU - Chen, Yi Ting

AU - Yu, Jau Song

AU - Chang, Yu Sun

PY - 2011

Y1 - 2011

N2 - This paper reports a negative-pressure driven microfluidic chip, which is capable of rapidly detecting bladder cancer bi-omarker, APOA1, with aid of magnetic beads-based Enzyme-linked immunosorbent assays (ELISA). Fluid can be handled through vacuum force and normally-closed valve. Comparing the experimental results between integrated chip and 96 well plate, the former requires less time (4 hr to 30 min), fewer reagent consumption (100 μl to 30 μl). In this study, the beads-based ELISA in chip has higher detection range and shows better performance in both sensitivity and linearity(99.2%) than conventional ELISA in plate.

AB - This paper reports a negative-pressure driven microfluidic chip, which is capable of rapidly detecting bladder cancer bi-omarker, APOA1, with aid of magnetic beads-based Enzyme-linked immunosorbent assays (ELISA). Fluid can be handled through vacuum force and normally-closed valve. Comparing the experimental results between integrated chip and 96 well plate, the former requires less time (4 hr to 30 min), fewer reagent consumption (100 μl to 30 μl). In this study, the beads-based ELISA in chip has higher detection range and shows better performance in both sensitivity and linearity(99.2%) than conventional ELISA in plate.

KW - Apolipoprotein A-I (APOA1)

KW - Bladder cancer

KW - ELISA

KW - Magnetic beads

KW - Microfluidic chip

UR - https://www.scopus.com/pages/publications/84883812485

M3 - 会议稿件

AN - SCOPUS:84883812485

SN - 9781618395955

T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

SP - 1692

EP - 1694

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

Chen YJ, Lin YH, Lai CS, Chen YT , Yu JS, Chang YS. A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1692-1694. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).

A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer

Abstract

Publication series

Conference

UN SDGs

Keywords

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