@inproceedings{735aa2ec9e1545b0a57347296a8e1647,
title = "A system enabling isolation and electrical property characterization of circulating tumour cells",
abstract = "This paper presents a system capable of characterizing electrical property of circulating tumour cells (CTCs) based on microfluidic impedance spectroscopy. Following negative selection, which consisted of red blood cell (RBC) lysis and CD45 depletion, suspected CTCs were left behind. Using a capillary tube, cells were then transferred directly into the entrance of the microfluidic device, and aspirated into a constriction channel for the quantification of specific membrane capacitance (Cspecific membrane) and cytoplasm conductivity (scytoplasm). Significant differences were found both in Cspecificmembrane and scytoplasmamong CTCs isolated from patients suffering from hepatic (Cspecific membrane=2.26 ± 0.69 μF/cm2and scytoplasm=0.88 ± 0.43 S/m, ncell=239), oral (1.82 ± 0.56 μF/cm2and 1.16 ± 0.49 S/m, ncell=269) and lung (1.55 ± 0.35 μF/cm2and 1.39 ± 0.69 S/m, ncell=468) cancers. These results validated the feasibility of the developed system, which may serve as a platform for the characterization of CTCs.",
keywords = "CTCs, Cytoplasm conductivity, Electrical property, Specific membrane capacitance",
author = "Chiu, {T. K.} and Y. Zhao and Chen, {D. Y.} and Hsieh, {C. H.} and K. Wang and Chou, {W. P.} and Fan, {B. Y.} and Wang, {J. B.} and J. Chen and Wu, {M. H.}",
year = "2016",
language = "英语",
series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
publisher = "Chemical and Biological Microsystems Society",
pages = "293--294",
booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",
}
