Abstract
BACKGROUND/AIM: A recent study suggested that solute carrier family 35 member A2 (SLC35A2) is related to poor prognosis in patients with breast cancer. SLC35A2 transports uridine diphosphate-galactose from the cytosol to the lumen of the endoplasmic reticulum and Golgi.
MATERIALS AND METHODS: Immunohistochemical expression of SLC35A2 was evaluated using tissue microarrays. Cell growth, migration, and invasion of breast cancer cells were examined following loss- and gain-of-expression of SLC35A2.
RESULTS: Normal breast tissue exhibited SLC35A2 immunoreactivity in the nucleus. A progressive increase in cytoplasmic expression from in situ carcinoma to invasive carcinoma was observed. There was a correlation between cytoplasmic SLC35A2 expression and breast cancer stage (p<0.001). MDA-MB-468 and MCF-7 cells transfected with SLC35A2 shRNA had unchanged cell viability but significantly reduced cell migration and invasion. In contrast, MDA-MB-231 and HCC1806 cells transfected with the SLC35A2 expression vector showed increased migration.
CONCLUSION: Breast cancer progression is accompanied by differential expression patterns of SLC35A2. The migratory or invasive capacity of breast cancer cells is associated with SLC35A2 expression.
| Original language | English |
|---|---|
| Pages (from-to) | 262-269 |
| Number of pages | 8 |
| Journal | In Vivo |
| Volume | 37 |
| Issue number | 1 |
| DOIs | |
| State | Published - 01 2023 |
Bibliographical note
Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.Keywords
- SLC35A2
- breast cancer
- immunohistochemistry
- Cell Movement/genetics
- Breast/pathology
- Breast Neoplasms/pathology
- Humans
- Gene Expression Regulation, Neoplastic
- Carcinoma/genetics
- Neoplasm Invasiveness/genetics
- MCF-7 Cells
- Cell Line, Tumor
- Female
- Cell Proliferation/genetics
