Aberrant transcripts of the cyclin-dependent kinase-associated protein phosphatase in hepatocellular carcinoma

C. T. Yeh*, S. C. Lu, T. C. Chen, C. Y. Peng, Y. F. Liaw

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

44 Scopus citations

Abstract

The cyclin-dependent kinase (Cdk)-associated protein phosphatase (KAP) is a human dual specificity protein phosphatase that dephosphorylates Cdk2 on threonine 160 in a cyclin-dependent manner. To investigate whether mutations of this enzyme occur in hepatocellular carcinoma (HCC), KAP mRNA was analyzed by reverse transcription-PCR (RT-PCR), followed by cloning and sequencing. Eight of 14 biopsy tissues obtained from advanced HCC, 6 of 13 surgically removed HCC tissues, and 2 of the adjacent noncancerous tissues contained aberrant KAP transcripts. Using the yeast two-hybrid system, five of seven representative KAP mutants were shown to be defective in interacting with Cdk2. These data suggest a possible role of KAP mutations in multiple-step hepatocarcinogenesis.

Original languageEnglish
Pages (from-to)4697-4700
Number of pages4
JournalCancer Research
Volume60
Issue number17
StatePublished - 01 09 2000
Externally publishedYes

Keywords

  • Amino Acid Sequence
  • CDC2-CDC28 Kinases
  • Carcinoma, Hepatocellular/enzymology
  • Carcinoma, Hepatocellular/genetics
  • Cell Cycle Proteins
  • Cyclin-Dependent Kinase 2
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Cyclin-Dependent Kinases/metabolism
  • Dual-Specificity Phosphatases
  • Female
  • Hepatitis B, Chronic/enzymology
  • Hepatitis B, Chronic/genetics
  • Hepatitis C, Chronic/enzymology
  • Hepatitis C, Chronic/genetics
  • Humans
  • Liver Neoplasms/enzymology
  • Liver Neoplasms/genetics
  • Liver/enzymology
  • Liver/physiology
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Mutation
  • Protein Tyrosine Phosphatases/biosynthesis
  • Protein Tyrosine Phosphatases/genetics
  • Protein Tyrosine Phosphatases/metabolism
  • Protein-Serine-Threonine Kinases/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism

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