Aberrant transcripts of the cyclin-dependent kinase-associated protein phosphatase in hepatocellular carcinoma

C. T. Yeh; S. C. Lu; T. C. Chen; C. Y. Peng; Y. F. Liaw

Aberrant transcripts of the cyclin-dependent kinase-associated protein phosphatase in hepatocellular carcinoma

C. T. Yeh^*
, S. C. Lu
, T. C. Chen
, C. Y. Peng
, Y. F. Liaw

^*Corresponding author for this work

Chang Gung Memorial Hospital

Research output: Contribution to journal › Journal Article › peer-review

44 Scopus citations

Abstract

The cyclin-dependent kinase (Cdk)-associated protein phosphatase (KAP) is a human dual specificity protein phosphatase that dephosphorylates Cdk2 on threonine 160 in a cyclin-dependent manner. To investigate whether mutations of this enzyme occur in hepatocellular carcinoma (HCC), KAP mRNA was analyzed by reverse transcription-PCR (RT-PCR), followed by cloning and sequencing. Eight of 14 biopsy tissues obtained from advanced HCC, 6 of 13 surgically removed HCC tissues, and 2 of the adjacent noncancerous tissues contained aberrant KAP transcripts. Using the yeast two-hybrid system, five of seven representative KAP mutants were shown to be defective in interacting with Cdk2. These data suggest a possible role of KAP mutations in multiple-step hepatocarcinogenesis.

Original language	English
Pages (from-to)	4697-4700
Number of pages	4
Journal	Cancer Research
Volume	60
Issue number	17
State	Published - 01 09 2000
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Amino Acid Sequence
CDC2-CDC28 Kinases
Carcinoma, Hepatocellular/enzymology
Carcinoma, Hepatocellular/genetics
Cell Cycle Proteins
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinase Inhibitor Proteins
Cyclin-Dependent Kinases/metabolism
Dual-Specificity Phosphatases
Female
Hepatitis B, Chronic/enzymology
Hepatitis B, Chronic/genetics
Hepatitis C, Chronic/enzymology
Hepatitis C, Chronic/genetics
Humans
Liver Neoplasms/enzymology
Liver Neoplasms/genetics
Liver/enzymology
Liver/physiology
Male
Middle Aged
Molecular Sequence Data
Mutation
Protein Tyrosine Phosphatases/biosynthesis
Protein Tyrosine Phosphatases/genetics
Protein Tyrosine Phosphatases/metabolism
Protein-Serine-Threonine Kinases/metabolism
RNA, Messenger/genetics
RNA, Messenger/metabolism

Cite this

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title = "Aberrant transcripts of the cyclin-dependent kinase-associated protein phosphatase in hepatocellular carcinoma",

abstract = "The cyclin-dependent kinase (Cdk)-associated protein phosphatase (KAP) is a human dual specificity protein phosphatase that dephosphorylates Cdk2 on threonine 160 in a cyclin-dependent manner. To investigate whether mutations of this enzyme occur in hepatocellular carcinoma (HCC), KAP mRNA was analyzed by reverse transcription-PCR (RT-PCR), followed by cloning and sequencing. Eight of 14 biopsy tissues obtained from advanced HCC, 6 of 13 surgically removed HCC tissues, and 2 of the adjacent noncancerous tissues contained aberrant KAP transcripts. Using the yeast two-hybrid system, five of seven representative KAP mutants were shown to be defective in interacting with Cdk2. These data suggest a possible role of KAP mutations in multiple-step hepatocarcinogenesis.",

keywords = "Amino Acid Sequence, CDC2-CDC28 Kinases, Carcinoma, Hepatocellular/enzymology, Carcinoma, Hepatocellular/genetics, Cell Cycle Proteins, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinase Inhibitor Proteins, Cyclin-Dependent Kinases/metabolism, Dual-Specificity Phosphatases, Female, Hepatitis B, Chronic/enzymology, Hepatitis B, Chronic/genetics, Hepatitis C, Chronic/enzymology, Hepatitis C, Chronic/genetics, Humans, Liver Neoplasms/enzymology, Liver Neoplasms/genetics, Liver/enzymology, Liver/physiology, Male, Middle Aged, Molecular Sequence Data, Mutation, Protein Tyrosine Phosphatases/biosynthesis, Protein Tyrosine Phosphatases/genetics, Protein Tyrosine Phosphatases/metabolism, Protein-Serine-Threonine Kinases/metabolism, RNA, Messenger/genetics, RNA, Messenger/metabolism",

author = "Yeh, \{C. T.\} and Lu, \{S. C.\} and Chen, \{T. C.\} and Peng, \{C. Y.\} and Liaw, \{Y. F.\}",

year = "2000",

month = sep,

day = "1",

language = "英语",

volume = "60",

pages = "4697--4700",

journal = "Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research Inc.",

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TY - JOUR

T1 - Aberrant transcripts of the cyclin-dependent kinase-associated protein phosphatase in hepatocellular carcinoma

AU - Yeh, C. T.

AU - Lu, S. C.

AU - Chen, T. C.

AU - Peng, C. Y.

AU - Liaw, Y. F.

PY - 2000/9/1

Y1 - 2000/9/1

N2 - The cyclin-dependent kinase (Cdk)-associated protein phosphatase (KAP) is a human dual specificity protein phosphatase that dephosphorylates Cdk2 on threonine 160 in a cyclin-dependent manner. To investigate whether mutations of this enzyme occur in hepatocellular carcinoma (HCC), KAP mRNA was analyzed by reverse transcription-PCR (RT-PCR), followed by cloning and sequencing. Eight of 14 biopsy tissues obtained from advanced HCC, 6 of 13 surgically removed HCC tissues, and 2 of the adjacent noncancerous tissues contained aberrant KAP transcripts. Using the yeast two-hybrid system, five of seven representative KAP mutants were shown to be defective in interacting with Cdk2. These data suggest a possible role of KAP mutations in multiple-step hepatocarcinogenesis.

AB - The cyclin-dependent kinase (Cdk)-associated protein phosphatase (KAP) is a human dual specificity protein phosphatase that dephosphorylates Cdk2 on threonine 160 in a cyclin-dependent manner. To investigate whether mutations of this enzyme occur in hepatocellular carcinoma (HCC), KAP mRNA was analyzed by reverse transcription-PCR (RT-PCR), followed by cloning and sequencing. Eight of 14 biopsy tissues obtained from advanced HCC, 6 of 13 surgically removed HCC tissues, and 2 of the adjacent noncancerous tissues contained aberrant KAP transcripts. Using the yeast two-hybrid system, five of seven representative KAP mutants were shown to be defective in interacting with Cdk2. These data suggest a possible role of KAP mutations in multiple-step hepatocarcinogenesis.

KW - Amino Acid Sequence

KW - CDC2-CDC28 Kinases

KW - Carcinoma, Hepatocellular/enzymology

KW - Carcinoma, Hepatocellular/genetics

KW - Cell Cycle Proteins

KW - Cyclin-Dependent Kinase 2

KW - Cyclin-Dependent Kinase Inhibitor Proteins

KW - Cyclin-Dependent Kinases/metabolism

KW - Dual-Specificity Phosphatases

KW - Female

KW - Hepatitis B, Chronic/enzymology

KW - Hepatitis B, Chronic/genetics

KW - Hepatitis C, Chronic/enzymology

KW - Hepatitis C, Chronic/genetics

KW - Humans

KW - Liver Neoplasms/enzymology

KW - Liver Neoplasms/genetics

KW - Liver/enzymology

KW - Liver/physiology

KW - Male

KW - Middle Aged

KW - Molecular Sequence Data

KW - Mutation

KW - Protein Tyrosine Phosphatases/biosynthesis

KW - Protein Tyrosine Phosphatases/genetics

KW - Protein Tyrosine Phosphatases/metabolism

KW - Protein-Serine-Threonine Kinases/metabolism

KW - RNA, Messenger/genetics

KW - RNA, Messenger/metabolism

UR - https://www.scopus.com/pages/publications/0034282513

M3 - 文章

C2 - 10987270

AN - SCOPUS:0034282513

SN - 0008-5472

VL - 60

SP - 4697

EP - 4700

JO - Cancer Research

JF - Cancer Research

IS - 17

ER -

Aberrant transcripts of the cyclin-dependent kinase-associated protein phosphatase in hepatocellular carcinoma

Abstract

UN SDGs

Keywords

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