Accumulation of a heat shock-like protein during differentiation of human erythroid cell line K562

Mandaleshwar K. Singh*, John Yu

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

55 Scopus citations

Abstract

The human erythroid cell line K562 provides a model system for studying erythroid differentiation and eukaryotic gene regulation. These cells express glycophorin A, spectrin and i antigen1-4. They accumulate embryonic and fetal haemoglobins on induction of erythroid differentiation with haemin, sodium butyrate or hydroxyurea5,6. In the present study, the protein composition of K562 cells during haemin-mediated induction of erythroid maturation was analysed by two-dimensional gel electrophoresis. Under conditions in which haemin did not affect cell viability and proliferation7, a protein of ∼70,000 molecular weight (MW) accumulated in the differentiated K562 cells. The accumulation appears to be due to an increase in the rate of RNA synthesis for this protein. The protein is related in sequence to a 70,000-MW heat shock protein. An antigenically related protein was also demonstrated in human bone marrow and accumulates at particular stages of human erythroid maturation.

Original languageEnglish
Pages (from-to)631-633
Number of pages3
JournalNature
Volume309
Issue number5969
DOIs
StatePublished - 1984
Externally publishedYes

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