Accurate detection of binary toxin producer from Clostridium difficile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

S.-F. Kuo; T.-L. Wu; H.-L. You; C.-C. Chien; J.-H. Chia; Chih-Hung Lee

doi:10.1016/j.diagmicrobio.2015.07.013

Accurate detection of binary toxin producer from Clostridium difficile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

S.-F. Kuo, T.-L. Wu, H.-L. You, C.-C. Chien, J.-H. Chia, Chih-Hung Lee

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

Abstract

Thirsty-six binary toxin producers were detected with 2 genotypes (cdtA⁺ and cdtB⁺ ) among 265 Clostridium difficile isolates by multiplex PCR. The rate of accurate differentiation between these 2 genotypes was 100% by 6-peak cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry.

Original language	American English
Pages (from-to)	229-231
Journal	Diagnostic Microbiology and Infectious Disease
Volume	83
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2015.07.013
State	Published - 2015

Keywords

Binary toxin
Binary toxin
C. difficile
C. difficile
MALDI-TOF
MALDI-TOF

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10.1016/j.diagmicrobio.2015.07.013

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title = "Accurate detection of binary toxin producer from Clostridium difficile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry",

abstract = "Thirsty-six binary toxin producers were detected with 2 genotypes (cdtA+ and cdtB+ ) among 265 Clostridium difficile isolates by multiplex PCR. The rate of accurate differentiation between these 2 genotypes was 100\% by 6-peak cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry.",

keywords = "Binary toxin, Binary toxin, C. difficile, C. difficile, MALDI-TOF, MALDI-TOF",

author = "S.-F. Kuo and T.-L. Wu and H.-L. You and C.-C. Chien and J.-H. Chia and Chih-Hung Lee",

year = "2015",

doi = "10.1016/j.diagmicrobio.2015.07.013",

language = "American English",

volume = "83",

pages = "229--231",

journal = "Diagnostic Microbiology and Infectious Disease",

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AU - Kuo, S.-F.

AU - Wu, T.-L.

AU - You, H.-L.

AU - Chien, C.-C.

AU - Chia, J.-H.

AU - Lee, Chih-Hung

PY - 2015

Y1 - 2015

N2 - Thirsty-six binary toxin producers were detected with 2 genotypes (cdtA+ and cdtB+ ) among 265 Clostridium difficile isolates by multiplex PCR. The rate of accurate differentiation between these 2 genotypes was 100% by 6-peak cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry.

AB - Thirsty-six binary toxin producers were detected with 2 genotypes (cdtA+ and cdtB+ ) among 265 Clostridium difficile isolates by multiplex PCR. The rate of accurate differentiation between these 2 genotypes was 100% by 6-peak cluster analysis of spectra generated by Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry.

KW - Binary toxin

KW - C. difficile

KW - MALDI-TOF

U2 - 10.1016/j.diagmicrobio.2015.07.013

DO - 10.1016/j.diagmicrobio.2015.07.013

M3 - Journal Article

C2 - 26293008

SN - 0732-8893

VL - 83

SP - 229

EP - 231

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

Accurate detection of binary toxin producer from Clostridium difficile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Abstract

Keywords

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