TY - JOUR
T1 - Activation-induced cytidine deaminase-mediated sequence diversification is transiently targeted to newly integrated DNA substrates
AU - Shu, Yuan Yang
AU - Fugmann, Sebastian D.
AU - Gramlich, Hillary S.
AU - Schatz, David G.
PY - 2007/8/31
Y1 - 2007/8/31
N2 - The molecular features that allow activation-induced cytidine deaminase (AID) to target Ig and certain non-Ig genes are not understood, although transcription has been implicated as one important parameter. We explored this issue by testing the mutability of a non-Ig transcription cassette in Ig and non-Ig loci of the chicken B cell line DT40. The cassette did not act as a stable long term mutation target but was able to be mutated in an AID-dependent manner for a limited time post-integration. This indicates that newly integrated DNA has molecular characteristics that render it susceptible to modification by AID, with implications for how targeting and mis-targeting of AID occurs.
AB - The molecular features that allow activation-induced cytidine deaminase (AID) to target Ig and certain non-Ig genes are not understood, although transcription has been implicated as one important parameter. We explored this issue by testing the mutability of a non-Ig transcription cassette in Ig and non-Ig loci of the chicken B cell line DT40. The cassette did not act as a stable long term mutation target but was able to be mutated in an AID-dependent manner for a limited time post-integration. This indicates that newly integrated DNA has molecular characteristics that render it susceptible to modification by AID, with implications for how targeting and mis-targeting of AID occurs.
UR - http://www.scopus.com/inward/record.url?scp=34548512009&partnerID=8YFLogxK
U2 - 10.1074/jbc.M704231200
DO - 10.1074/jbc.M704231200
M3 - 文章
C2 - 17613522
AN - SCOPUS:34548512009
SN - 0021-9258
VL - 282
SP - 25308
EP - 25313
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 35
ER -