TY - JOUR
T1 - Adipose-Derived Mesenchymal Stem Cell Exosomes Suppress Hepatocellular Carcinoma Growth in a Rat Model
T2 - Apparent Diffusion Coefficient, Natural Killer T-Cell Responses, and Histopathological Features
AU - Ko, Sheung Fat
AU - Yip, Hon Kan
AU - Zhen, Yen Yi
AU - Lee, Chen Chang
AU - Lee, Chia Chang
AU - Huang, Chung Cheng
AU - Ng, Shu Hang
AU - Lin, Jui Wei
N1 - Publisher Copyright:
© 2015 Sheung-Fat Ko et al.
PY - 2015
Y1 - 2015
N2 - We sought to evaluate the effects of adipose-derived mesenchymal stem cells (ADMSCs) exosomes on hepatocellular carcinoma (HCC) in rats using apparent diffusion coefficient (ADC), natural killer T-cell (NKT-cell) responses, and histopathological features. ADMSC-derived exosomes appeared as nanoparticles (30-90 nm) on electron microscopy and were positive for CD63, tumor susceptibility gene-101, and β-catenin on western blotting. The control (n=8) and exosome-treated (n=8) rats with N1S1-induced HCC underwent baseline and posttreatment day 10 and day 20 magnetic resonance imaging and measurement of ADC. Magnetic resonance imaging showed rapidly enlarged HCCs with low ADCs in the controls. The exosome-treated rats showed partial but nonsignificant tumor reduction, and significant ADC and ADC ratio increases on day 10. On day 20, the exosome-treated rats harbored significantly smaller tumors and volume ratios, higher ADC and ADC ratios, more circulating and intratumoral NKT-cells, and low-grade HCC (P<0.05 for all comparisons) compared to the controls. The ADC and volume ratios exhibited significant inverse correlations (P<0.001, R2=0.679). ADMSC-derived exosomes promoted NKT-cell antitumor responses in rats, thereby facilitating HCC suppression, early ADC increase, and low-grade tumor differentiation. ADC may be an early biomarker of treatment response.
AB - We sought to evaluate the effects of adipose-derived mesenchymal stem cells (ADMSCs) exosomes on hepatocellular carcinoma (HCC) in rats using apparent diffusion coefficient (ADC), natural killer T-cell (NKT-cell) responses, and histopathological features. ADMSC-derived exosomes appeared as nanoparticles (30-90 nm) on electron microscopy and were positive for CD63, tumor susceptibility gene-101, and β-catenin on western blotting. The control (n=8) and exosome-treated (n=8) rats with N1S1-induced HCC underwent baseline and posttreatment day 10 and day 20 magnetic resonance imaging and measurement of ADC. Magnetic resonance imaging showed rapidly enlarged HCCs with low ADCs in the controls. The exosome-treated rats showed partial but nonsignificant tumor reduction, and significant ADC and ADC ratio increases on day 10. On day 20, the exosome-treated rats harbored significantly smaller tumors and volume ratios, higher ADC and ADC ratios, more circulating and intratumoral NKT-cells, and low-grade HCC (P<0.05 for all comparisons) compared to the controls. The ADC and volume ratios exhibited significant inverse correlations (P<0.001, R2=0.679). ADMSC-derived exosomes promoted NKT-cell antitumor responses in rats, thereby facilitating HCC suppression, early ADC increase, and low-grade tumor differentiation. ADC may be an early biomarker of treatment response.
UR - http://www.scopus.com/inward/record.url?scp=84939796146&partnerID=8YFLogxK
U2 - 10.1155/2015/853506
DO - 10.1155/2015/853506
M3 - 文章
AN - SCOPUS:84939796146
SN - 1687-9678
VL - 2015
JO - Stem Cells International
JF - Stem Cells International
M1 - 853506
ER -