Adsorption behaviors of nucleic acid by cationic magnetic nanoparticles

Chi Hsien Liu*, Yi Fan Hsien

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Cationic magnetic nanoparticles are prepared by covalently binding spermidine and polyethylenimine onto the surface of nanoparticles via a glutaraldehyde coupling method. Nanoparticles modified by spermidine or polyethylenimine were characterized using Fourier-transformed infrared spectra, transmission electron microscopy and scanning electron microscopy. In this study, cationic magnetic nanoparticles were prepared by covalently conjugating cationic ligands onto the surface of nanoparticles. The plasmid DNA adsorption by the surface-modified magnetic nanoparticles was analyzed by Freundlich, Temkin, and Langmuir models. The maximal adsorption capacities in Langmuir model for polyethylenimine- and spermidine- modified nanoparticles are 341 and 116 μg/mg, respectively. Overall, the results demonstrated that the polyethylenimine-modified magnetic nanoparticles has the potential for purification of plasmid.

Original languageEnglish
Title of host publicationInnovation for Applied Science and Technology
Pages271-275
Number of pages5
DOIs
StatePublished - 2013
Event2nd International Conference on Engineering and Technology Innovation 2012, ICETI 2012 - Kaohsiung, Taiwan
Duration: 02 11 201206 11 2012

Publication series

NameApplied Mechanics and Materials
Volume284-287
ISSN (Print)1660-9336
ISSN (Electronic)1662-7482

Conference

Conference2nd International Conference on Engineering and Technology Innovation 2012, ICETI 2012
Country/TerritoryTaiwan
CityKaohsiung
Period02/11/1206/11/12

Keywords

  • Magnetic nanoparticles
  • Plasmid
  • Polyethylenimine
  • Spermidine

Fingerprint

Dive into the research topics of 'Adsorption behaviors of nucleic acid by cationic magnetic nanoparticles'. Together they form a unique fingerprint.

Cite this