TY - JOUR
T1 - Aldehyde-mannan antigen complexes target the MHC class I antigen-presentation pathway
AU - Apostolopoulos, Vasso
AU - Pietersz, Geoffrey A.
AU - Gordon, Siamon
AU - Martinez-Pomares, Luisa
AU - McKenzie, Lan F.C.
PY - 2000
Y1 - 2000
N2 - Antigens such as MUC1 coupled to oxidized mannan lead to rapid and efficient MHC class I presentation to CD8+ cells and a preferential T1 response; after reduction there is class II presentation and a T2 immune response. We now show that the selective advantage of the oxidized mannan-MUC1 is due to the presence of aldehydes and not Schiff bases, and that oxidized mannan-MUC1 binds to the mannose and not scavenger receptors and is internalized and presented by MHC class I molecules 1000 times more efficiently than when reduced. After internalization there is rapid access to the class I pathway via endosomes but not lysosomes, proteasomal processing and transport to the endoplasmic reticulum, Golgi apparatus and cell surface. Aldehydes cause rapid entry into the class I pathway, and can therefore direct the subsequent immune response.
AB - Antigens such as MUC1 coupled to oxidized mannan lead to rapid and efficient MHC class I presentation to CD8+ cells and a preferential T1 response; after reduction there is class II presentation and a T2 immune response. We now show that the selective advantage of the oxidized mannan-MUC1 is due to the presence of aldehydes and not Schiff bases, and that oxidized mannan-MUC1 binds to the mannose and not scavenger receptors and is internalized and presented by MHC class I molecules 1000 times more efficiently than when reduced. After internalization there is rapid access to the class I pathway via endosomes but not lysosomes, proteasomal processing and transport to the endoplasmic reticulum, Golgi apparatus and cell surface. Aldehydes cause rapid entry into the class I pathway, and can therefore direct the subsequent immune response.
KW - Aldehyde
KW - Antigen presentation
KW - Cytotoxic T lymphocyte
KW - Immunotherapy
KW - Mannose receptor
UR - http://www.scopus.com/inward/record.url?scp=0034035702&partnerID=8YFLogxK
U2 - 10.1002/1521-4141(200006)30:6<1714::AID-IMMU1714>3.0.CO;2-C
DO - 10.1002/1521-4141(200006)30:6<1714::AID-IMMU1714>3.0.CO;2-C
M3 - 文章
C2 - 10898509
AN - SCOPUS:0034035702
SN - 0014-2980
VL - 30
SP - 1714
EP - 1723
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 6
ER -