Alkaline phosphatase activity during differentiation of the human promyelocytic leukemia cell line, HL-60

Lillian L.L. Wei; Eric K.W. Hui; J. S. Wei; Benjamin Y.M. Yung

doi:10.1016/0006-291X(92)91758-I

Alkaline phosphatase activity during differentiation of the human promyelocytic leukemia cell line, HL-60

Lillian L.L. Wei, Eric K.W. Hui, J. S. Wei, Benjamin Y.M. Yung^*

^*Corresponding author for this work

Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

13 Scopus citations

Abstract

The differentiation of HL-60 promyelocytic cells toward mature myelocytic cells induced by retinoic acid (RA) was accompanied by a quantitative similar increase in alkaline phosphatase (ALP) activity. The potentiation of RA-induced differentiation and the enhancement of ALP activity by sphinganine seemed to correlate with each other. The combination of RA and sphinganine increased in parallel the percentage of mature cells and the ALP activities. Short exposures (4-8h) of HL-60 cells to RA promoted differentiation and ALP activity to a fraction (about 50%) of their maximums which were achieved in cells after 24h or longer RA exposure. Our results indicate that the key step for the induction of ALP activity and the differentiation by RA probably takes place within 24h of incubation.

Original language	English
Pages (from-to)	487-494
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	182
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)91758-I
State	Published - 31 01 1992

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title = "Alkaline phosphatase activity during differentiation of the human promyelocytic leukemia cell line, HL-60",

abstract = "The differentiation of HL-60 promyelocytic cells toward mature myelocytic cells induced by retinoic acid (RA) was accompanied by a quantitative similar increase in alkaline phosphatase (ALP) activity. The potentiation of RA-induced differentiation and the enhancement of ALP activity by sphinganine seemed to correlate with each other. The combination of RA and sphinganine increased in parallel the percentage of mature cells and the ALP activities. Short exposures (4-8h) of HL-60 cells to RA promoted differentiation and ALP activity to a fraction (about 50%) of their maximums which were achieved in cells after 24h or longer RA exposure. Our results indicate that the key step for the induction of ALP activity and the differentiation by RA probably takes place within 24h of incubation.",

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T1 - Alkaline phosphatase activity during differentiation of the human promyelocytic leukemia cell line, HL-60

AU - Wei, Lillian L.L.

AU - Hui, Eric K.W.

AU - Wei, J. S.

AU - Yung, Benjamin Y.M.

PY - 1992/1/31

Y1 - 1992/1/31

N2 - The differentiation of HL-60 promyelocytic cells toward mature myelocytic cells induced by retinoic acid (RA) was accompanied by a quantitative similar increase in alkaline phosphatase (ALP) activity. The potentiation of RA-induced differentiation and the enhancement of ALP activity by sphinganine seemed to correlate with each other. The combination of RA and sphinganine increased in parallel the percentage of mature cells and the ALP activities. Short exposures (4-8h) of HL-60 cells to RA promoted differentiation and ALP activity to a fraction (about 50%) of their maximums which were achieved in cells after 24h or longer RA exposure. Our results indicate that the key step for the induction of ALP activity and the differentiation by RA probably takes place within 24h of incubation.

AB - The differentiation of HL-60 promyelocytic cells toward mature myelocytic cells induced by retinoic acid (RA) was accompanied by a quantitative similar increase in alkaline phosphatase (ALP) activity. The potentiation of RA-induced differentiation and the enhancement of ALP activity by sphinganine seemed to correlate with each other. The combination of RA and sphinganine increased in parallel the percentage of mature cells and the ALP activities. Short exposures (4-8h) of HL-60 cells to RA promoted differentiation and ALP activity to a fraction (about 50%) of their maximums which were achieved in cells after 24h or longer RA exposure. Our results indicate that the key step for the induction of ALP activity and the differentiation by RA probably takes place within 24h of incubation.

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AN - SCOPUS:0026597420

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Alkaline phosphatase activity during differentiation of the human promyelocytic leukemia cell line, HL-60

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