Allopurinol blocks shock-wave-induced rises in cytosolic calcium levels in MDCK cells

C. R. Jan*, W. C. Chen, Y. H. Lee, J. K. Huang, H. C. Ou, C. J. Tseng

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

5 Scopus citations

Abstract

Allopurinol has been reported to ameliorate the side effects in patients following shock wave lithotripsy (SWL); however, the mechanism has not been studied. We have examined the protective effect of allopurinol on Madin- Darby canine kidney (MDCK) cells after shock wave exposure (SWE) by determining the release of aspartate aminotransferase (ASAT) and lactate dehydrogenase (LD), and the resting cytosolic Ca2+ concentration ([Ca2+](i)). In SWE-treated cells, the release of ASAT and LD increased immediately, but largely transiently, by approximately 23% and 5-fold over control, respectively. Within 1-6 h after SWE there was a gradual rise in the resting [Ca2+](i) of 16-137% above control. Allopurinol did not affect the transient enzyme release but blocked the long-term rises in the resting [Ca2+](i). The transient changes in [Ca2+](i) evoked by two hormones, ATP and bradykinin, and a drug that releases Ca2+ from internal Ca2+ stores, thapsigargin, were only slightly affected in allopurinol-treated cells. We conclude that the protection conferred by allopurinol on patients treated with SWL might involve a direct protection of the kidney cells by maintaining a normal resting [Ca2+](i).

Original languageEnglish
Pages (from-to)427-432
Number of pages6
JournalUrological Research
Volume25
Issue number6
DOIs
StatePublished - 12 1997
Externally publishedYes

Keywords

  • Allopurinol
  • Calcium signalling
  • Lithotripsy
  • MDCK cells
  • Shock waves

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