Alpha-fetoprotein detection by using a localized surface plasmon coupled fluorescence fiber-optic biosensor

Chien Chou*, Ying Feng Chang, Ran Chou Chen, Ying Chang Li, Chih Jen Yu, Bao Yu Hsieh

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Alpha-fetoprotein (AFP) detection by using a localized surface plasmon coupled fluorescence (LSPCF) fiber-optic biosensor is setup and experimentally demonstrated. It is based on gold nanoparticle (GNP) and coupled with localized surface plasmon wave on the surface of GNP. In this experiment, the fluorophores are labeled on anti-AFP which are bound to protein A conjugated GNP. Thus, LSPCF is excited with high efficiency in the near field of localized surface plasmon wave. Therefore, not only the sensitivity of LSPCF biosensor is enhanced but also the specific selectivity of AFP is improved. Experimentally, the ability of real time measurement in the range of AFP concentration from 0.1ng/ml to 100ng/ml was detected. To compare with conventional methods such as enzyme-linked immunosorbent assay (ELISA) or radioimmunoassay (RIA), the LSPCF fiber-optic biosensor performs higher or comparable detection sensitivity, respectively.

Original languageEnglish
Title of host publicationOptics in Health Care and Biomedical Optics III
DOIs
StatePublished - 2008
Externally publishedYes
EventOptics in Health Care and Biomedical Optics III - Beijing, China
Duration: 12 11 200715 11 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6826
ISSN (Print)1605-7422

Conference

ConferenceOptics in Health Care and Biomedical Optics III
Country/TerritoryChina
CityBeijing
Period12/11/0715/11/07

Keywords

  • AFP
  • Fiber-optic biosensor
  • Gold nanoparticles
  • Localized surface plasmon

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