Alteration of the copy number and deletion of mitochondrial DNA in human hepatocellular carcinoma

P. H. Yin, H. C. Lee, G. Y. Chau, Y. T. Wu, S. H. Li, W. Y. Lui, Y. H. Wei, T. Y. Liu, C. W. Chi*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

195 Scopus citations

Abstract

Somatic mutations in mitochondrial DNA (mtDNA) have been detected in hepatocellular carcinoma (HCC). However, it remains unclear whether mtDNA copy number and mitochondrial biogenesis are altered in HCC. In this study, we found that mtDNA copy number and the content of mitochondrial respiratory proteins were reduced in HCCs as compared with the corresponding non-tumorous livers. MtDNA copy number was significantly reduced in female HCC but not in male HCC. Expression of the peroxisome proliferator-activated receptor γ coactivator-1 was significantly repressed in HCCs (P<0.005), while the expression of the mitochondrial single-strand DNA-binding protein was upregulated, indicating that the regulation of mitochondria biogenesis is disturbed in HCC. Moreover, 22% of HCCs carried a somatic mutation in the mtDNA D-loop region. The non-tumorous liver of the HCC patients with a long-term alcohol-drinking history contained reduced mtDNA copy number (P<0.05) and higher level of the 4977 bp-deleted mtDNA (P<0.05) as compared with non-alcohol patients. Our results suggest that reduced mtDNA copy number, impaired mitochondrial biogenesis and somatic mutations in mtDNA are important events during carcinogenesis of HCC, and the differential alterations in mtDNA of male and female HCC may contribute to the differences in the clinical manifestation between female and male HCC patients.

Original languageEnglish
Pages (from-to)2390-2396
Number of pages7
JournalBritish Journal of Cancer
Volume90
Issue number12
DOIs
StatePublished - 14 06 2004
Externally publishedYes

Keywords

  • Alcohol
  • Gender
  • Hepatoma
  • Mitochondrial biogenesis
  • Somatic mutation

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