Alterations of oxygen consumption and extracellular acidification rates by glutamine in PBMCs of SLE patients

Hui Ting Lee, Chen Sung Lin, Siao Cian Pan, Tsai Hung Wu, Chyou Shen Lee, Deh Ming Chang, Chang Youh Tsai*, Yau Huei Wei

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

20 Scopus citations

Abstract

We evaluated plasma glutamine levels and basal mitochondrial oxygen consumption rate (mOCR B ) and basal extracellular acidification rate (ECAR B ) of peripheral blood mononuclear cells (PBMCs) of systemic lupus erythematous (SLE) patients and healthy controls (HCs). Lower plasma glutamine levels correlated with higher SLE disease activity indexes (p = 0.025). Incubated in DMEM containing 100 mg/dL glucose, SLE-PBMCs displayed lower mOCR B (p = 0.018) but similar ECAR B (p = 0.467) to those of HC-PBMCs, and their mOCR B got elevated (p < 0.001) without altering ECAR B (p = 0.239) by supplementation with 2 or 4 mM glutamine. We conclude that impaired mitochondrial respiration of SLE-PBMCs could be improved by glutamine under euglycemic condition.

Original languageEnglish
Pages (from-to)65-74
Number of pages10
JournalMitochondrion
Volume44
DOIs
StatePublished - 01 2019
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018

Keywords

  • Extracellular acidification rate (ECAR)
  • Glutamine
  • Oxygen consumption rate (OCR)
  • Peripheral blood mononuclear cells (PBMCs)
  • Systemic lupus erythematous (SLE)

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