Amphiphilic photocleavable block copolymers based on monomethyl poly(ethylene glycol) and poly(4-substituted-ε-caprolactone): Synthesis, characterization, and cellular uptake

Kang Yu Peng, Shiu Wei Wang, Mu Yi Hua, Ren Shen Lee*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

9 Scopus citations

Abstract

This paper presents the synthesis of a novel amphiphilic block copolymer containing photodegradable linkers as junction points between hydrophilic and hydrophobic chains. MPEG-ONB-PXCL block copolymers were synthesized by a combination of ROP and "clicks" from a difunctional photoresponsive initiator (ONB). The copolymers are biodegradable and biocompatible, and can self-assemble into photoresponsive micelles. When polymer solutions were exposed to UV, we observed significant changes in the structure and morphology of particles. Fluorescence emission measurements showed that Nile red (NR), a hydrophobic dye, encapsulated by the MPEG-ONB-PXCL micelles, is released upon irradiation because of disruption of the micelles. Light-triggered bursts were observed for Indomethacin (IMC)-loaded MPEG-ONB-PXCL micelles upon at 30 min of light irradiation. No significant toxicity of these nanoparticles was found, at concentrations up to 1000 μg mL-1, before or after light irradiation. The doxorubicin (DOX)-loaded micelles facilitated improved uptake of DOX by HeLa cells within 60 min, and were retained primarily in the cytoplasm.

Original languageEnglish
Pages (from-to)18453-18463
Number of pages11
JournalRSC Advances
Volume3
Issue number40
DOIs
StatePublished - 28 10 2013

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